NON-TRYPTOPHAN FLUORESCENCE ASSOCIATED WITH HUMAN LENS PROTEIN - APPARENT COMPLEXITY AND ISOLATION OF BITYROSINE AND ANTHRANILIC ACID
NON-TRYPTOPHAN FLUORESCENCE ASSOCIATED WITH HUMAN LENS PROTEIN - APPARENT COMPLEXITY AND ISOLATION OF BITYROSINE AND ANTHRANILIC ACID
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DOI：
10.1016/0014-4835(78)90132-x
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发表时间：
1978-01-01
影响因子：
3.4
通讯作者：
SPECTOR, A
中科院分区：
文献类型：
--
作者：
GARCIACASTINEIRAS, S;DILLON, J;SPECTOR, A
为了表征人白内障晶状体中与年龄相关的蛋白质结合荧光化合物，对不溶性黄色蛋白质组分进行蛋白水解消化，然后使用离子交换、凝胶过滤和高压液相色谱进行分级。荧光成分的复杂且异质的混合物与这些晶状体蛋白相关。 3,3''-二酪氨酸和邻氨基苯甲酸在结构上被鉴定为蛋白水解产物的成分。后一种化合物似乎是不稳定前体的降解产物。
To characterize the age-related protein bound fluorescent compounds of human cataractous lenses, the insoluble yellow protein component was proteolytically digested and then fractionated using ion-exchange, gel filtration and high pressure liquid chromatography. A complex and heterogeneous mixture of fluorescent components was associated with these lens proteins. 3,3''-Bityrosine and anthranilic acid were structurally identified as constituents of the proteolytic hydrolysate. The latter compound appeared to be a degradation product of an unstable precursor.