Cell labeling for magnetic resonance imaging with the T1 agent manganese chloride
Cell labeling for magnetic resonance imaging with the T1 agent manganese chloride
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DOI：
10.1002/nbm.1000
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发表时间：
2006-02-01
影响因子：
2.9
通讯作者：
Koretsky, AP
中科院分区：
文献类型：
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作者：
Aoki, I;Yoshiyuki, T;Koretsky, AP
人们对使用核磁共振技术追踪细胞迁移越来越感兴趣。到目前为止，这一领域的大部分工作都是使用超细的氧化铁颗粒进行的。免疫细胞很难用氧化铁颗粒标记。过继输注肿瘤特异性T细胞和N细胞到肿瘤微环境的能力可能是它们治疗效果的关键决定因素。我们测试了这样的假设，即淋巴细胞和B细胞会用MnCl2标记到可以用T-1加权MRI检测到的水平。0.05-1.0 mM的MnCl2与人淋巴细胞孵育1h后，淋巴细胞的信号显著增强。用碘化丙啶和Annexin V染色进行的流式细胞仪检测显示，在1.0 mM的MnCl2作用1h后，淋巴细胞在即刻和24小时内没有发生凋亡或坏死。重要的是，NK细胞和细胞毒性T细胞在与高达0.5 mM的MnCl2孵育后仍保持其体外杀伤能力。这是第一个描述用MnCl2标记淋巴细胞的报告。我们的数据表明，在基于MRI的细胞迁移研究中，MnCl2可能是氧化铁细胞标记的替代方法。版权所有(C)2006 John Wiley&Sons，Ltd.
There is growing interest in using MRI to track cellular migration. To date, most work in this area has been performed using ultra-small particles of iron oxide. Immune cells are difficult to label with iron oxide particles. The ability of adoptively infused tumor specific T cells and N cells to traffic to the tumor microenvironment may be a critical determinant of their therapeutic efficacy. We tested the hypothesis that lymphocytes and B cells would label with MnCl2 to a level that would allow their detection by T-1-weighted MRI. Significant signal enhancement was observed in human lymphocytes after a 1 h incubation with 0.05-1.0mM MnCl2. A flow cytometry-based evaluation using propidium iodide and Annexin V staining showed that lymphocytes did not undergo apoptosis or necrosis immediately after and 24h following a 1 h incubation with up to 1.0 mM MnCl2. Importantly, NK cells and cytotoxic T cells maintained their ill vitro killing capacity after being incubated with up to 0.5 mM MnCl2. This is the first report to describe the use of MnCl2 to label lymphocytes. Our data suggests MnCl2 might be an alternative to iron oxide cell labeling for MRI-based cell migration studies. Copyright (c) 2006 John Wiley & Sons, Ltd.