Novel cellular evidence of lipophagy within the Sertoli cells during spermatogenesis in the turtle.
Novel cellular evidence of lipophagy within the Sertoli cells during spermatogenesis in the turtle.
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乌龟精子发生过程中支持细胞内脂肪吞噬的新细胞证据
DOI：
10.18632/aging.101070
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发表时间：
2016-10-16
期刊：
影响因子：
--
通讯作者：
Chen Q
中科院分区：
文献类型：
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作者：
Ahmed N;Liu Y;Chen H;Yang P;Waqas Y;Liu T;Gandahi JA;Huang Y;Wang L;Song X;Rajput IR;Wang T;Chen Q
精子发生是产生单倍体精子的复杂过程，Sertoli细胞内脂质液滴（LDS）的形成对于维持正常的精子发生至关重要。但是，在Sertoli细胞中LDS的利用仍然在很大程度上未知。在本研究中，精子细胞的增殖已于5月开始，而减数分裂细胞主要发生在7月，大多数精子生成细胞在10月的生成小管中观察到。然而，TEM和油红O染色表明，与10月相比，5月相比，Sertoli细胞内积累了更多的LDS。分离膜/吞噬体附着几个LDS，这表明LDS可能是自噬体生物发生的内源能量的来源。 LDS于5月被封闭在自噬体中，而自噬体和线粒体在10月在Sertoli细胞中直接与大型LDS相连。此外，免疫组织化学结果表明，与10月相比，LC3在Sertoli细胞上的定位更强。这项研究是第一个为Sertoli细胞内脂质消耗的两种不同模式提供的明确证据，这是精子发生过程中Sertoli生殖细胞通信的关键方面。
Spermatogenesis is a complex process producing haploid spermatozoa, and the formation of lipid droplets (LDs) within Sertoli cells is critical to maintaining normal spermatogenesis. However, the utilization of LDs within Sertoli cells is still largely unknown. In the present study, proliferation of spermatogonial cells had begun in May, whereas the meiotic cells occurred predominately in July and majority of spermiogenic cells were observed in the seminiferous tubules in October. However, TEM and Oil Red O staining demonstrated that a larger number of LDs had accumulated within the Sertoli cells in May compared to that in October. There were several LDs attached to the isolation membrane/phagophore, suggesting that the LDs may be a source of endogenous energy for the biogenesis of autophagosomes. The LDs were enclosed within the autophagosomes in May, whereas, autophagosomes and mitochondria were directly attached with large LDs within the Sertoli cells in October. Furthermore, immunohistochemistry results demonstrated the stronger localization of LC3 on the Sertoli cells in May than in October. This study is the first to provide clear evidence of the two different modes of lipophagy for lipid consumption within Sertoli cells, which is a key aspect of Sertoli germ cell communication during spermatogenesis.