Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells.
Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells.
复制标题
Cdc20在哺乳动物细胞中的动力学和中心体的CDC20的快速微管动力学。
DOI：
10.1083/jcb.200201135
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发表时间：
2002-09-02
影响因子：
7.8
通讯作者：
Gorbsky, Gary J
中科院分区：
文献类型：
--
作者：
Kallio, Marko J;Beardmore, Victoria A;Weinstein, Jasminder;Gorbsky, Gary J
CDC20是后期促进复合体/环体(APC/C)的底物适配器和激活剂，APC/C是E3泛素连接酶，其活性是后期启动和退出有丝分裂所必需的。一种绿色荧光蛋白衍生物，CDC20-GFP，在整个细胞周期中与中心体结合，并从早期晚期到末期晚期与动粒结合。我们绘制了与动点和中心体关联所需的CDC20的不同结构域。FRAP测量显示，在动心(t1/2=5.1 S)和纺锤体极(t1/2=4.7S)处表现出极快的动力学。这种快速的周转不依赖于微管。CDC20通过动点的快速传递可能确保在未连接/松弛的动点处的纺锤体检查点信号可以连续地抑制APC/CCdc20靶向整个细胞的后期抑制物(Securins)，直到所有的染色体都正确地附着在有丝分裂纺锤体上。
Cdc20 is a substrate adaptor and activator of the anaphase-promoting complex/cyclosome (APC/C), the E3 ubiquitin ligase whose activity is required for anaphase onset and exit from mitosis. A green fluorescent protein derivative, Cdc20–GFP, bound to centrosomes throughout the cell cycle and to kinetochores from late prophase to late telophase. We mapped distinct domains of Cdc20 that are required for association with kinetochores and centrosomes. FRAP measurements revealed extremely rapid dynamics at the kinetochores (t 1/2 = 5.1 s) and spindle poles (t 1/2 = 4.7 s). This rapid turnover is independent of microtubules. Rapid transit of Cdc20 through kinetochores may ensure that spindle checkpoint signaling at unattached/relaxed kinetochores can continuously inhibit APC/CCdc20 targeting of anaphase inhibitors (securins) throughout the cell until all the chromosomes are properly attached to the mitotic spindle.