The best method of detecting prior Helicobacter pylori infection
The best method of detecting prior Helicobacter pylori infection
复制标题
DOI：
10.3748/wjg.v11.i36.5672
复制
发表时间：
2005-09-28
影响因子：
4.3
通讯作者：
Wu, Deng-Chyang
中科院分区：
文献类型：
--
作者：
Lu, Chien-Yu;Kuo, Chao-Hung;Wu, Deng-Chyang
目的：先前幽门螺杆菌（幽门螺杆菌）感染经常被低估。这些低估误导了试图确定幽门螺杆菌与某些胃肠道病变（如肠化生、萎缩性胃炎和胃癌）之间意义的医生。本研究尝试使用新开发的免疫印迹试剂盒Helico Blot 2.1准确、简便、快速地检测过去的幽门螺杆菌感染。方法：33例患者，其中25例幽门螺杆菌感染，8例未感染。所有患者均接受连续的胃镜检查和c -13-尿素呼吸试验（UBT），每隔6或12个月进行一次，持续4年。同时采集每位患者的血清样本。确认胃内幽门螺杆菌感染符合金标准。25名幽门螺杆菌感染的患者在最初的细菌确认后接受了三联治疗，并成功根除了感染。采用Helico Blot 2.1检测连续获得的血清。结果：Helico Blot 2.1检测到的当前感染标志物对当前幽门螺杆菌感染不可靠。幽门螺杆菌35和37 ku抗体在治愈1年后血清转换率显著。随访4年，116 ku（细胞毒素相关抗原[CagA]）和Helico Blot 2.1血清阳性率接近100%。CagA抗原和Helico blot 2.1均可作为长期幽门螺杆菌感染的指标。结论：Helico Blot 2.1可检出长达4年的幽门螺杆菌感染，是迄今为止检出长期幽门螺杆菌感染的最佳方法。(C) 2005 WJG出版社和爱思唯尔公司。版权所有。
AIM: Prior Helicobacter pylori (H pylori) infection has often been underestimated. These underestimations have misled physicians attempting to determine the significance between H pylori and certain gastrointestinal lesions such as intestinal metaplasia, atrophic gastritis, and gastric cancer. Our study endeavored to detect past H pylori infections accurately, easily, and rapidly with the newly developed immunoblot kit, Helico Blot 2.1.METHODS: Thirty-three patients, including 25 H pylori infected and 8 uninfected cases, were enrolled in our study. All patients received consecutive gastroendoscopic examinations and C-13-urea breath test (UBT) tests at 6- or 12-mo intervals for up to 4 years. Serum samples were obtained from each patient at the same time. Intragastric H pylori infection was confirmed in accordance with the gold standard. Twenty-five H pylori-infected patients received triple therapies after initial bacterial confirmation, and were successful in eradicating their infections. Serially obtained sera were tested by means of Helico Blot 2.1.RESULTS: Current infection marker detected by Helico Blot 2.1 was unreliable for representing ongoing H pylori infection. Only 35 and 37 ku antibodies of H pylori had significant seroconversion rates 1 year after having been cured. The seropositive rates of 116 ku (cytotoxin-associated antigen [CagA]) and Helico Blot 2.1 were nearly 100% during 4-year follow-up period. Both CagA antigen and Helico blot 2.1 could serve as indicators of long-term H pylori infection.CONCLUSION: Helico Blot 2.1 can detect past H pylori infections for up to 4 years, and is the best method to date for detecting previous long-term H pylori infection. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.