RCA Strands as Scaffolds To Create Nanoscale Shapes by a Few Staple Strands
RCA Strands as Scaffolds To Create Nanoscale Shapes by a Few Staple Strands
复制标题
RCA 股线作为支架，通过几股短纤线创建纳米级形状
DOI：
10.1021/ja400102s
复制
发表时间：
2013-02-27
影响因子：
15
通讯作者：
Xiao, Shou-Jun
中科院分区：
文献类型：
--
作者：
Ma, Yinzhou;Zheng, Hongning;Xiao, Shou-Jun
通过滚环扩增 (RCA) 生成的数千个核苷酸 (nt) 长的单链 DNA 被用作支架，通过遵循折纸设计原理（以 1.5 圈交叉）来创建带有一些短链的 DNA 纳米级线和板。环状模板的核心序列，用于通过RCA产生数十和数百个串联重复的拷贝，是根据Seeman的核酸结构工程序列设计原理设计的(Seeman, N. C. J. Biomol. Struct. Dyn. 1990, 8, 573)。将RCA产品折叠成纳米级形状的意义在于订书钉和支架链代码的设计灵活性、一些短订书钉链折叠RCA产品的周期序列的简单性以及较低的成本。
Thousands of nucleotide(nt)-long single strand DNAs, generated from rolling-circle-amplification (RCA), were used as scaffolds to create DNA nanoscale wires and plates with a few short staple strands by following the origami design principle with a crossover at 1.5 turns. The core sequence of the circle template, for producing tens and hundreds of tandemly repeated copies of it by RCA, was designed according to Seeman's sequence design principle for nucleic acid structural engineering (Seeman, N. C. J. Biomol. Struct. Dyn. 1990, 8, 573). The significance for folding the RCA products into nanoscale shapes lies in the design flexibility of both staple and scaffold strand codes, simplicity of a few short staple strands to fold the periodic sequence of RCA products, and lower cost.