Real-time RT-PCR for norovirus screening in shellfish
Real-time RT-PCR for norovirus screening in shellfish
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DOI：
10.1016/j.jviromet.2004.08.023
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发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Le Guyader, FS
中科院分区：
文献类型：
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作者：
Loisy, F;Atmar, RL;Le Guyader, FS
实时荧光定量RT-PCR结合病毒特异性扩增子的扩增和检测，是一种很有前途的工具，用于检测环境或食品样品中的诺如病毒，如贝类。我们建立了一种基于一步检测的实时RT-PCR检测方法，使用单引物和探针的诺如病毒基因组I和M 70和7个RT-PCR单位的基因组I和II参考诺如病毒株，分别检测到人工污染的牡蛎。对150种存档的自然污染贝类的新方法验证证实了基因组II引物组可用于检测自1995年以来在法国传播的大量不同菌株，但基因组I菌株很少被检测到。(C)2004 Elsevier B. V.保留所有权利。
Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and M Seventy and seven RT-PCR units of genogroup I and II reference norovirus strains, respectively, were detected in artificially contaminated oysters. Validation of the new method on 150 archived naturally contaminated shellfish confirmed the utility of the genogroup II primer set to detect a large range of different strains circulating in France since 1995, but genogroup I strains were detected infrequently. (C) 2004 Elsevier B.V. All rights reserved.