Development of a bone nonunion in a noncritical segmental tibia defect model in sheep utilizing interlocking nail as an internal fixation system
Development of a bone nonunion in a noncritical segmental tibia defect model in sheep utilizing interlocking nail as an internal fixation system
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DOI：
10.1016/j.jss.2013.02.060
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发表时间：
2013-08-01
影响因子：
2.2
通讯作者：
Juarez-Mosqueda, M. Lourdes
中科院分区：
文献类型：
--
作者：
Lozada-Gallegos, Angel R.;Letechipia-Moreno, Jorge;Juarez-Mosqueda, M. Lourdes
背景：骨不连是骨折修复的重要并发症。在小动物物种上开发的现有模型防止使用设计用于植入人骨中的骨接合材料。本研究的目的是开发一个非关键节段性胫骨缺损的绵羊，一个物种的大小类似于human.Materials和方法：动物被分为两组，每组4只动物的不愈合过程。在第1组（实验组）中，通过手术剥离胫骨远端截骨边缘的骨膜，保持边缘间隔5 mm，并在差距中放置不完整的O形硅胶环来创建缺损。第2组（对照组）对胫骨远端简单骨折进行干预。两组均使用交锁髓内钉作为固定系统。结果：对照组骨缺损修复过程典型，术后8周行X线片、组织学及形态计量学检查。相反，实验组显示骨折线边缘圆整，骨痂形成稀少。骨痂显示骨形成量减少和纤维组织含量大（P = 0.001）。结论：这些结果表明，我们的模型开发了一个萎缩性骨不连的绵羊，一个物种具有多种相似性，人类，如重量，大小，骨结构，骨重建过程。(c)2013 Elsevier Inc. All rights reserved.
Background: The bone nonunion is an important complication of bone fracture repair. The existing models developed on small animal species prevent using osteosynthesis materials designed to be implanted in human bones. The goal of this study was to develop a nonunion process in a noncritical segmental tibial defect in sheep, a species analogous in size to humans.Materials and methods: The animals were divided into two groups of four animals each. In Group 1 (experimental), the defect was created by surgically stripping the periosteum from the edges of a distal tibial osteotomy, keeping the edges 5 mm apart, and placing an incomplete O-shaped silicone ring in the gap. Group 2 (control) was intervened with a simple fracture at the distal end of the tibia. In both groups an interlocking nail was used as a fixation system. Over 8 wk after surgery, radiographs and histologic and histomorphometric analyses were performed.Results: The control group showed a typical bone repair process. In contrast, the experimental group showed a fracture line with rounded edges and a scarce callus formation. The bone callus showed reduced amount of bone formation and large content of fibrous tissue (P = 0.001).Conclusions: These results indicate that our model developed an atrophic nonunion in sheep, a species having multiple similarities to humans, such as weight, size, bone structure, and bone remodeling process. (c) 2013 Elsevier Inc. All rights reserved.