Transcriptional responses in neonate and adult Daphnia magna in relation to relative susceptibility to genotoxicants
Transcriptional responses in neonate and adult Daphnia magna in relation to relative susceptibility to genotoxicants
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DOI：
10.1016/j.aquatox.2011.04.016
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发表时间：
2011-08-01
影响因子：
4.5
通讯作者：
Chipman, J. Kevin
中科院分区：
文献类型：
--
作者：
David, Rhiannon M.;Dakic, Vanja;Chipman, J. Kevin
尽管在生态毒性测试中已确定使用水蚤大型水蚤，但关于低等动物对基因毒性化学品的反应或其检测基因毒性化学品的敏感性的信息很少，特别是在不同的生命阶段。开发了彗星测定方法并将其应用于水蚤细胞，但仅发现对基因毒剂重铬酸钠（0.2-1μM）、金苏啶（0.1-2μM）以及苯并芘（BaP）和重铬酸钠的混合物（从0.01μM BaP和0.1μM重铬酸钠到0.25μM BaP和0.75μM重铬酸钠）。使用 Agilent D. magna 寡核苷酸微阵列进行转录组分析，以评估重铬酸钠和 BaP 混合物（旨在产生加合和氧化 DNA）对基因转录的影响。新生儿（
Little information is available on the responses of lower animals to genotoxic chemicals or on their sensitivity for detecting genotoxic chemicals, especially at different life-stages, despite the established use of the water flea Daphnia magna in ecotoxicity testing. Comet assay methodology was developed and applied to daphnid cells but only limited, non-statistically significant responses to the genotoxicants sodium dichromate (0.2-1 mu M), chrysoidine (0.1-2 mu M), and mixtures of benzo-a-pyrene (BaP) and sodium dichromate were found (from 0.01 mu M BaP & 0.1 mu M sodium dichromate to 0.25 mu M BaP & 0.75 mu M sodium dichromate). Transcriptomic analyses using Agilent D. magna oligonucleotide microarrays were undertaken to assess the effect of a mixture of sodium dichromate and BaP (designed to produce both adducted and oxidised DNA) on gene transcription. Neonates (