Staphylococcal protein A primed leukocytes enhance the autologous mixed lymphocyte reaction.
Staphylococcal protein A primed leukocytes enhance the autologous mixed lymphocyte reaction.
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葡萄球菌蛋白 A 引发的白细胞增强自体混合淋巴细胞反应。
DOI：
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发表时间：
1986
影响因子：
3.1
通讯作者：
J. Ellner
中科院分区：
文献类型：
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作者：
G. Berk;M. Lederman;M. L. Liebman;J. Ellner
将人外周血单核细胞 (PBMC) 在单独的培养基中或与各种有丝分裂原一起预孵育 3 天，然后洗涤和照射。然后将预孵育的细胞与自体 T 细胞一起在自体混合淋巴细胞反应 (AMLR) 中培养。葡萄球菌蛋白 A (SPA) 预处理 PBMC 可增强自体 T 淋巴细胞增殖，从 1375 +/- 321 cpm（平均值 +/- SEM 未处理的 PBMC）增加到 42,467 +/- 7,985 cpm（SPA 引发的 PBMC）（p 小于 0.01）。 SPA 处理的 PBMC 增强 AMLR 的能力不仅仅反映了它们在预培养中的增殖，因为用植物血凝素和刀豆球蛋白 A 预培养的 PBMC 显示出比 SPA 处理的 PBMC 更大的增殖，但仅最小程度地增强了 AMLR。动力学研究和 PBMC 预暴露于分级剂量的伽马辐射表明，SPA 增强 AMLR 是由动力学和放射敏感性不同的 2 个成分介导的。尽管 PBMC 与 SPA 一起孵育并没有增加具有可检测表面 Ia 抗原的细胞百分比，但 SPA 确实增加了预孵育细胞中 Ia 的密度。细胞分离研究表明，SPA 对 AMLR 的增强不是由 T 细胞介导的，而是由非粘附的非 E 玫瑰花结部分细胞介导的。 AMLR 的 SPA 增强与刺激物群体中 Ia 密度的增加有关，但与 Ia 阳性细胞的增加无关，并且由增殖依赖性和增殖非依赖性机制介导。
Human peripheral blood mononuclear cells (PBMC) were preincubated for 3 days in medium alone or with various mitogens then washed and irradiated. The preincubated cells then were cultured with autologous T-cells in an autologous mixed lymphocyte reaction (AMLR). Staphylococcal protein A (SPA) pretreatment of PBMC enhanced autologous T-lymphocyte proliferation from 1375 +/- 321 cpm (mean +/- SEM untreated PBMC) to 42,467 +/- 7,985 cpm (SPA primed PBMC) (p less than 0.01). The ability of SPA treated PBMC to enhance the AMLR was not simply a reflection of their proliferation in preculture, as PBMC precultured with phytohemagglutinin and concanavalin A showed greater proliferation than SPA-treated PBMC yet only minimally enhanced the AMLR. Kinetic studies and pre-exposure of PBMC to graded doses of gamma radiation showed that SPA augmentation of the AMLR was mediated by 2 components which differed in kinetics and radiosensitivity. Although incubation of PBMC with SPA did not increase the percentage of cells with detectable surface Ia antigen, SPA did increase the density of Ia in the preincubated cells. Cell separation studies revealed that SPA enhancement of the AMLR was not mediated by T-cells, but was mediated by a non-adherent non-E-rosetting fraction of cells. SPA enhancement of the AMLR was associated with an increased Ia density in the stimulator population but not with an increase in Ia positive cells and was mediated by proliferation-dependent and proliferation-independent mechanisms.
人类自体混合白细胞反应 (MLR) 的进一步表征。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
MacDermott,RP;Stacey,MC
通讯作者：
Stacey,MC
激活自体反应性辅助 T 淋巴细胞以分化人 B 淋巴细胞。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Shin,HS;Wang,CY;Choi,YS
通讯作者：
Choi,YS