In vitro comparison of the biological activity of alumina ceramic and titanium particles associated with aseptic loosening
In vitro comparison of the biological activity of alumina ceramic and titanium particles associated with aseptic loosening
复制标题
氧化铝陶瓷和钛颗粒与无菌松动相关的生物活性的体外比较
DOI：
10.1088/1748-6041/7/4/045019
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发表时间：
2012-08-01
影响因子：
4
通讯作者：
Huang, Dong-sheng
中科院分区：
文献类型：
--
作者：
Ding, Yue;Qin, Chu-qiang;Huang, Dong-sheng
假体磨损颗粒被认为在假体周围骨溶解的发生和发展中发挥着核心作用，从而导致假体无菌性松动。本研究旨在比较与颗粒引起的无菌性关节松动相关的陶瓷颗粒和钛颗粒的生物活性。制备不同尺寸的氧化铝陶瓷颗粒和钛颗粒刺激小鼠巨噬细胞RAW 264.7，通过qPCR和ELISA检测不同时间点肿瘤坏死因子α（TNF-α）和核因子κB配体受体激活剂（RANKL）的表达。在所有颗粒存在的情况下，TNF-α的表达以时间依赖性方式增加，而RANKL的表达没有表现出规则的表达模式。值得注意的是，较小尺寸的颗粒比较大尺寸的颗粒引发的 TNF-α 和 RANKL 水平显着更高。与钛颗粒相比，陶瓷颗粒引起的 TNF-α 产量显着降低。因此，钛和氧化铝陶瓷颗粒的生物活性与颗粒的尺寸成反比，并且RANKL的表达与TNF-α的表达不平行。陶瓷对陶瓷人工关节假体的成功可能归因于陶瓷颗粒的低生物活性，如此处所证明。
Prosthetic wear particles are thought to play a central role in the initiation and development of periprosthetic osteolysis, leading to aseptic loosening of prostheses. This study aimed to compare the biological activity of ceramic and titanium particles that are associated with particle-induced, aseptic joint loosening. Different sizes of alumina-ceramic particles and titanium particles were prepared to stimulate murine macrophage cells RAW 264.7, of which the expressions of tumor necrosis factor alpha (TNF-alpha) and receptor activator of nuclear factor-kappa B ligand (RANKL) were measured by qPCR and ELISA at various time points. In the presence of all particles, the expression of TNF-alpha increased in a time-dependent manner, whereas the expression of RANKL showed no regular expression patterns. Notably, particles of smaller sizes provoked significantly higher levels of TNF-alpha and RANKL than those of larger sizes. Compared to the titanium particles, the ceramic particles provoked a significantly lower production of TNF-alpha. Thus, the bioactivities of titanium and alumina ceramic particles were inversely proportional to the sizes of the particles, and the expression of RANKL was not parallel to that of TNF-alpha. The successful outcome of ceramic-on-ceramic artificial joint prostheses may be attributed to the low biological activity of ceramic particles, as evidenced here.