A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery
A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery
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大肠杆菌 K12 的 spc 操纵子中先前未识别的基因指定了蛋白质输出机制的一个组成部分
DOI：
10.1016/0092-8674(82)90422-6
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发表时间：
1982
期刊：
影响因子：
64.5
通讯作者：
S. Emr
中科院分区：
文献类型：
--
作者：
J. Shultz;T. Silhavy;M. Berman;N. Fiil;S. Emr
基因prlA编码一种似乎在大肠杆菌中蛋白质输出中起作用的因子。该结论基于改变prlA基因产物的突变恢复具有缺陷信号序列的包膜蛋白的输出的发现。先前的结果表明，prlA基因位于已知编码十种不同核糖体蛋白的操纵子（spc）中。我们的研究表明，pr/A基因位于该操纵子中最后一个已知的核糖体蛋白基因的启动子远端。来自体外构建的基因融合的证据表明，pr/A编码至少含有300个氨基酸的蛋白质。因此，一个迄今尚未确定的蛋白质指定的基因内的spc操纵子似乎是一个组成部分的细胞蛋白质输出机制。
The gene prlA codes for a factor that appears to function in the export of proteins in Escherichia coli. This conclusion is based on the finding that mutations altering the prlA gene product restore export of envelope proteins with defective signal sequences. Previous results showed that the prlA gene lies in an operon (spc) known to code for ten different ribosomal proteins. Our studies show that the pr/A gene lies promoter-distal to the last known ribosomal protein gene in this operon. Evidence from gene fusions constructed in vitro suggests that pr/A codes for a protein containing at least 300 amino acids. Thus a heretofore unidentified protein specified by a gene within the spc operon appears to be a component of the cellular protein export machinery.