Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance
Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance
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DOI：
10.1128/jcm.39.11.4037-4041.2001
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发表时间：
2001-11-01
影响因子：
9.4
通讯作者：
Méndez-Alvarez, S
中科院分区：
文献类型：
--
作者：
Pérez-Roth, E;Claverie-Martín, F;Méndez-Alvarez, S
在这项工作中，我们描述了一种多重PCR检测的临床相关的抗生素耐药基因窝藏的一些金黄色葡萄球菌菌株，并在物种水平上同时识别这些菌株。优化了条件，以分别从单个反应管中的单个菌落中同时检测mecA（编码高水平甲氧西林耐药性）、ileS-1（编码高水平莫匹罗星耐药性）和femB（编码甲氧西林耐药性必需因子）基因的310-、456-和651-bp区域。femB PCR片段可用于S.金黄色。使用50株耐甲氧西林S.金黄色葡萄球菌（MRSA）和适当的对照菌株。该检测方法可快速、简单、可行、特异、灵敏和准确地鉴定莫匹罗星耐药MRSA临床分离株，并可作为临床微生物学实验室的诊断检测系统应用，促进抗生素治疗的设计和使用。
In this work, we describe a multiplex PCR assay for the detection of clinically relevant antibiotic resistance genes harbored by some Staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. Conditions were optimized for the simultaneous detection of the 310-, 456-, and 651-bp regions of the mecA (encoding high-level methicillin resistance), ileS-1 (encoding high-level mupirocin resistance), and femB (encoding a factor essential for methicillin resistance) genes, respectively, from a single colony in a single reaction tube. The femB PCR fragment allows the specific identification of S. aureus. Validation of the method was performed using 50 human isolates of methicillin-resistant S. aureus (MRSA) and the appropriate control strains. This assay offers a rapid, simple, feasible, specific, sensitive, and accurate identification of mupirocin-resistant MRSA clinical isolates and could be systematically applied as a diagnostic test in clinical microbiology laboratories, facilitating the design and use of antibiotic therapy.