Increased expression of SDF-1/CXCR4 is associated with lymph node metastasis of invasive micropapillary carcinoma of the breast
Increased expression of SDF-1/CXCR4 is associated with lymph node metastasis of invasive micropapillary carcinoma of the breast
复制标题
SDF-1/CXCR4 表达增加与乳腺浸润性微乳头状癌淋巴结转移相关
DOI：
10.1111/j.1365-2559.2009.03289.x
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发表时间：
2009-05-01
期刊：
影响因子：
6.4
通讯作者：
Fu, Li
中科院分区：
文献类型：
--
作者：
Liu, Fangfang;Lang, Ronggang;Fu, Li
基质细胞衍生因子-1 （SDF-1）及其受体CXCR4参与肿瘤趋化和转移。目的是研究它们在乳腺浸润性微乳头状癌（IMPC）转移中的作用，这是一种高倾向于淋巴结扩散的肿瘤。我们通过免疫组织化学和化学原位杂交（CISH）比较了103例含有IMPC成分的乳腺癌中SDF-1和CXCR4的表达与96例未指定类型的浸润性导管癌（IDC）的对照组。结果显示，SDF-1和CXCR4在IMPC成分的肿瘤细胞中的优势细胞质表达高于非IMPC成分和对照IDC病例，且与阳性淋巴结数显著相关（P < 0.05）。细胞膜上的SDF-1表达在IMPC中较IDC少（P = 0.021）。淋巴血管内皮细胞中SDF-1的免疫组化检测在IMPC中更为常见（P = 0.007），且与淋巴结状态显著相关（P = 0.002），尽管CISH很少检测到SDF-1 mRNA。本研究提示胞质中SDF-1/CXCR4表达上调可能是促进IMPC淋巴结转移的分子机制之一。
Stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 are implicated in tumour chemotaxis and metastasis. The aim was to examine their roles in the metastasis of invasive micropapillary carcinoma (IMPC) of the breast, a tumour with a high propensity for nodal spread.We compared the expression of SDF-1 and CXCR4 in 103 cases of breast cancer containing IMPC components with a control group of 96 cases of invasive ductal carcinoma (IDC), not otherwise specified type by immunohistochemistry and chemical in situ hybridization (CISH). The results showed that the predominant cytoplasmic expression of both SDF-1 and CXCR4 was greater in tumour cells of the IMPC components than in those of the non-IMPC components and the control IDC cases, and was correlated significantly with the number of positive lymph nodes (P < 0.05). SDF-1 expression on cell membranes was less frequently identified in IMPC than IDC (P = 0.021). Immunohistochemical detection of SDF-1 in endothelial cells of lymphatic vessels was more common in IMPC (P = 0.007) and correlated significantly with lymph node status (P = 0.002), although SDF-1 mRNA was rarely detected by CISH.This study suggests that up-regulation of cytoplasmic expression of SDF-1/CXCR4 might be one of the molecular mechanisms facilitating lymph node metastasis of IMPC.