Comparative analysis of adherence, viability, proliferation and morphology of umbilical cord tissue-derived mesenchymal stem cells seeded on different titanium-coated expanded polytetrafluoroethylene scaffolds
Comparative analysis of adherence, viability, proliferation and morphology of umbilical cord tissue-derived mesenchymal stem cells seeded on different titanium-coated expanded polytetrafluoroethylene scaffolds
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DOI：
10.1088/1748-6041/5/6/065004
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发表时间：
2010-12-01
影响因子：
4
通讯作者：
Eissner, Guenther
中科院分区：
文献类型：
--
作者：
Hollweck, Trixi;Marschmann, Michaela;Eissner, Guenther
脐带组织是间充质干细胞的一个有吸引力的新来源。脐带组织来源的间充质干细胞（UCMSC）具有自我更新、多能性和免疫天然性，并且它们可以在没有医学干预的情况下获得。将UCMSC转移到心脏的缺血区域可能对组织再生产生有利的影响。通过注射到梗塞区域的典型细胞递送的益处通常由于差的细胞保留和存活而受到限制。另一种给药途径是使用植入梗死区的填充支架。在本文中，UCMSC的接种效率未涂层和钛涂层膨胀聚四氟乙烯（ePTFE）支架具有不同的表面结构进行了测定。Dualmesh（R）（DM）提供了一个灯芯绒状的表面，与心血管补片（CVP）的相对平坦的表面形成对比。对UCMSC的粘附、存活和增殖的研究表明，钛涂层支架上级未涂层支架，与表面结构无关。显微图像显示接种在未涂覆支架上的球形UCMSC。相比之下，钛涂层支架上的UCMSC显示其特征性的纺锤形形态和CVP的均匀覆盖。总之，临床批准的CVP的钛涂层增强了UCMSC的保留，从而为受损心肌的修复提供了潜在的细胞递送系统。
Umbilical cord tissue comprises an attractive new source for mesenchymal stem cells. Umbilical cord tissue-derived mesenchymal stem cells (UCMSC) exhibit self-renewal, multipotency and immunological naivity, and they can be obtained without medical intervention. The transfer of UCMSC to the ischemic region of the heart may have a favorable impact on tissue regeneration. Benefit from typical cell delivery by injection to the infarcted area is often limited due to poor cell retention and survival. Another route of administration is to use populated scaffolds implanted into the infarcted zone. In this paper, the seeding efficiency of UCMSC on uncoated and titanium-coated expanded polytetrafluoroethylene (ePTFE) scaffolds with different surface structures was determined. Dualmesh (R) (DM) offers a corduroy-like surface in contrast to the comparatively planar surface of cardiovascular patch (CVP). The investigation of adherence, viability and proliferation of UCMSC demonstrates that titanium-coated scaffolds are superior to uncoated scaffolds, independent of the surface structure. Microscopic images reveal spherical UCMSC seeded on uncoated scaffolds. In contrast, UCMSC on titanium-coated scaffolds display their characteristic spindle-shaped morphology and a homogeneous coverage of CVP. In summary, titanium coating of clinically approved CVP enhances the retention of UCMSC and thus offers a potential cell delivery system for the repair of the damaged myocardium.