Analysis of the immunoglobulin heavy chain gene of secondary diffuse large B-cell lymphoma that subsequently developed in four cases with B-cell chronic lymphocytic leukemia or lymphoplasmacytoid lymphoma (Richter syndrome)
Analysis of the immunoglobulin heavy chain gene of secondary diffuse large B-cell lymphoma that subsequently developed in four cases with B-cell chronic lymphocytic leukemia or lymphoplasmacytoid lymphoma (Richter syndrome)
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DOI：
10.1046/j.1440-1827.2000.01094.x
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发表时间：
2000-08-01
影响因子：
2.2
通讯作者：
Abe, M
中科院分区：
文献类型：
--
作者：
Nakamura, N;Kuze, T;Abe, M
对四例 B 细胞 Richter 综合征病例的免疫球蛋白重链基因（IgH 基因）进行分析，以确定继发性弥漫性大 B 细胞淋巴瘤（DLBCL）是否可能与初始 B 细胞慢性淋巴细胞白血病（B-CLL）和淋巴浆细胞样淋巴瘤（LPL）来自同一克隆，或者是新发事件，以及继发性 DLBCL 是否表现出克隆内微异质性。两例初始 B-CLL 和继发性 DLBCL 均表达 CD5 抗原。对三例初始 B-CLL 或 LPL 和继发 DLBCL 的样本进行检查并进行比较。两例继发性DLBCL（CD5（+）病例和CD5（-）病例）聚合酶链式反应扩增IgH基因与初发B-CLL不同，揭示了新的恶性克隆。另一个病例（CD5（-））显示继发性DLBCL具有与初始LPL相同的序列，表明具有相同的克隆起源。继发性DLBCL的IgH基因可变区（CD5（+）2例和CD5（-）2例）表现出0.5-9.0％的体细胞突变范围，并且无克隆内微异质性。
The immunoglobulin heavy chain gene (IgH gene) was analysed in four cases of B-cell Richter syndrome, in order to determine whether a secondary diffuse large B-cell lymphoma (DLBCL) could arise from the same clone as the initial B-cell chronic lymphocytic leukemia (B-CLL) and lymphoplasmacytoid lymphoma (LPL) or be a de novo event, and whether secondary DLBCL shows an intraclonal microheterogeneity. Both the initial B-CLL and secondary DLBCL in two cases expressed CD5 antigen. Both samples of the initial B-CLL or LPL and the secondary DLBCL in three cases were examined for comparison. The polymerase chain reaction-amplified IgH gene of secondary DLBCL in two cases (CD5(+) case and CD5(-) case) were different from those of the initial B-CLL, revealing a new malignant clone. The other case (CD5(-)) showed that secondary DLBCL had a sequence identical to the initial LPL, indicating the same clonal origin. The variable region of the IgH gene of secondary DLBCL (CD5(+) two cases and CD5(-) two cases) exhibited a 0.5-9.0% somatic mutation range and no intraclonal microheterogeneity.