HISTOCHEMICAL-DEMONSTRATION OF HEAVY-METALS - A REVISED VERSION OF THE SULFIDE SILVER METHOD SUITABLE FOR BOTH LIGHT AND ELECTRON-MICROSCOPY
HISTOCHEMICAL-DEMONSTRATION OF HEAVY-METALS - A REVISED VERSION OF THE SULFIDE SILVER METHOD SUITABLE FOR BOTH LIGHT AND ELECTRON-MICROSCOPY
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DOI：
10.1007/bf00592566
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
DANSCHER, G
中科院分区：
文献类型：
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作者：
DANSCHER, G
研究了硫化银法的3个步骤：将金属转化为金属硫化物；固定并包埋或冷冻组织以进行切片；在物理显影剂中将金属银沉积在金属硫化物上。在此基础上，对修正后的方法进行了描述和讨论。尤其重要的是，在灌流过程中保持足够但低浓度的硫离子；避免使用氧化剂或酸固定剂；确保石蜡包埋或EPON聚合过程中的低温，以及使用慢效物理显影剂。给出了来自不同组织[大鼠、小鼠、豚鼠、刺猬和负鼠]的金属硫化物图案的例子。
The 3 steps of the sulfide silver method were examined; transformation of metals to metal sulfides; fixation and embedding or freezing of the tissue for sectioning; and deposition of metallic silver on the metal sulfides in a physical developer. Based on the results, a revised method is described and discussed. It is particularly important to maintain a sufficient but low concentration of sulfide ions during the perfusion; to avoid using oxidating or acid fixatives, to ensure low temperatures while embedding in paraffin or during polymerization of Epon and to use a slow-acting physical developer. Examples of the metal sulfide pattern from various tissues [rats, mice, guinea pigs, hedgehogs and opossums] are presented.