A magnetic-dependent protein corona of tailor-made superparamagnetic iron oxides alters their biological behaviors
A magnetic-dependent protein corona of tailor-made superparamagnetic iron oxides alters their biological behaviors
复制标题
定制的超顺磁性氧化铁的磁依赖性蛋白质冠改变了它们的生物行为
DOI：
10.1039/c5nr08447d
复制
发表时间：
2016-01-01
期刊：
影响因子：
6.7
通讯作者：
Gu, Zhongwei
中科院分区：
文献类型：
--
作者：
Liu, Ziyao;Zhan, Xiaohui;Gu, Zhongwei
In recent years, it is becoming increasingly evident that once nanoparticles come into contact with biological fluids, a protein corona surely forms and critically affects the biological behaviors of nanoparticles. Herein, we investigate whether the formation of protein corona on the surface of superparamagnetic iron oxides (SPIOs) is influenced by static magnetic field. Under static magnetic field, there is no obvious variation in the total amount of protein adsorption, but the proportion of adsorbed proteins significantly changes. Noticeably, certain proteins including apolipoproteins, complement system proteins and acute phase proteins, increase in the protein corona of SPIOs in the magnetic field. More importantly, the magnetic-dependent protein corona of SPIOs enhances the cellular uptake of SPIOs into the normal cell line (3T3 cells) and tumor cell line (HepG2 cells), due to increased adsorption of apolipoprotein. In addition, SPIOs with the magnetic-dependent protein corona cause high cytotoxicity to 3T3 cells and HepG2 cells. This work discloses that superparamagnetism as a key feature of SPIOs affects the composition of protein corona to a large extent, which further alters the biological behaviors of SPIOs.
