Mammalian transgenesis by intracytoplasmic sperm injection
Mammalian transgenesis by intracytoplasmic sperm injection
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DOI：
10.1126/science.284.5417.1180
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发表时间：
1999-05-14
期刊：
影响因子：
56.9
通讯作者：
Yanagimachi, R
中科院分区：
文献类型：
--
作者：
Perry, ACF;Wakayama, T;Yanagimachi, R
共注射未受精的小鼠卵母细胞与精子头和外源DNA编码的绿色荧光蛋白（GFP）或β-半乳糖苷酶报告产生64%至94%的转基因表达胚胎，反映DNA精子头协会共同注射前。非选择性地将GFP系列的胚胎转移到代孕母亲身上，产生了大约20%的表达整合转基因的后代。这些数据表明，外源性DNA可以重复地传递到一个卵母细胞的显微注射精子，并建议一个适应性强的转基因方法。
Coinjection of-unfertilized mouse oocytes with sperm heads and exogenous DNA encoding either a green fluorescent protein (GFP) or beta-galactosidase reporter produced 64 to 94 percent transgene-expressing embryos, reflecting DNA-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos in the GFP series generated about 20 percent offspring expressing the integrated transgene. These data indicate that exogenous DNA can reproducibly be delivered into an oocyte by microinjected spermatozoa and suggest an adaptable method of transgenesis.