Nanosensing at the single cell level.
Nanosensing at the single cell level.
复制标题
单细胞水平的纳米传感。
DOI：
10.1016/j.sab.2007.11.027
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Vo-Dinh,Tuan
中科院分区：
文献类型：
--
作者：
Vo-Dinh,Tuan
本文介绍了一个概述的发展，操作和应用的光学纳米生物传感器用于在体内检测生物靶标在单个活细胞。纳米生物传感器配备有固定的生物受体探针（例如，抗体、酶底物）对特定分子靶标具有选择性。激光激发被传输到纤维中，在纤维尖端产生渐逝场，以便激发结合到固定在纤维尖端的生物受体的靶分子。光度测量系统检测光信号（例如，荧光）来源于分析物分子或来源于分析物-生物受体反应。在活细胞中的细胞凋亡的生物物种和分子信号通路的检测的例子进行了讨论，以说明单细胞分析的纳米生物传感器技术的潜力。
This article presents an overview of the development, operation, and applications of optical nanobiosensors for use in in vivo detection of biotargets in individual living cells. The nanobiosensors are equipped with immobilized bioreceptor probes (e.g., antibodies, enzyme substrate) selective to specific molecular targets. Laser excitation is transmitted into the fiber producing an evanescent field at the tip of the fiber in order to excite target molecules bound to the bioreceptors immobilized at the fiber tips. A photometric system detects the optical signal (e.g., fluorescence) originated from the analyte molecules or from the analyte–bioreceptor reaction. Examples of detection of biospecies and molecular signaling pathways of apoptosis in a living cell are discussed to illustrate the potential of the nanobiosensor technology for single cell analysis.