Silencing of cZNF292 circular RNA suppresses human glioma tube formation via the Wnt/β-catenin signaling pathway.
Silencing of cZNF292 circular RNA suppresses human glioma tube formation via the Wnt/β-catenin signaling pathway.
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cZNF292 环状 RNA 的沉默通过 Wnt/β-catenin 信号通路抑制人神经胶质瘤管的形成
DOI：
10.18632/oncotarget.11523
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发表时间：
2016-09-27
期刊：
影响因子：
--
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Yang P;Qiu Z;Jiang Y;Dong L;Yang W;Gu C;Li G;Zhu Y
CircRNA是一种新型的RNA分子，它由共价闭合的环状结构组成，没有5′-3′极性，没有polyA尾，由于环状结构，相对稳定。因此，它们可能成为肿瘤治疗的潜在靶点和诊断生物标志物。cZNF 292是一种重要的环状致癌RNA，在管形成过程中起关键作用。本研究旨在探讨cZNF 292在人脑胶质瘤管形成中的作用及其可能的作用机制。我们发现cZNF 292沉默通过抑制胶质瘤细胞增殖和细胞周期进程来抑制管形成。人胶质瘤U87 MG和U251细胞的细胞周期进程通过Wnt/β-catenin信号通路和相关基因如PRR 11、Cyclin A、p-CDK 2、VEGFR-1/2、p-VEGFR-1/2和EGFR停止在S/G2/M期。结果表明，cZNF 292沉默在管形成过程中起重要作用，并有可能作为胶质瘤的治疗靶点和生物标志物。
CircRNA is a novel type of RNA molecule formed by a covalently closed loop which have no 5′-3′ polarity and possess no polyA tail and relatively stable due to the cyclic structure. Therefore, they may serve as potential targets and diagnosis biomarkers for tumor therapy. cZNF292 is an important circular oncogenic RNA and plays a critical role in the progression of tube formation. This study is aimed at exploring the role of cZNF292 in human glioma tube formation and its potential mechanism of action. We found that cZNF292 silencing suppresses tube formation by inhibiting glioma cell proliferation and cell cycle progression. Cell cycle progression in human glioma U87MG and U251 cells was halted at S/G2/M phase via the Wnt/β-catenin signaling pathway and related genes such as PRR11, Cyclin A, p-CDK2, VEGFR-1/2, p-VEGFR-1/2 and EGFR. The results suggest that cZNF292 silencing plays an important role in the tube formation process and has potential for application as a therapeutic target and biomarker in glioma.