Aerotaxis in Halobacterium salinarium is methylation-dependent.
Aerotaxis in Halobacterium salinarium is methylation-dependent.
复制标题
盐杆菌的趋气性是甲基化依赖性的。
DOI：
10.1099/13500872-141-11-2945
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Taylor,BL
中科院分区：
文献类型：
--
作者：
Lindbeck,JC;GoulbourneJr,EA;Johnson,MS;Taylor,BL
对氧气梯度的行为反应（趋氧性）已经在古细菌盐生盐杆菌（Halobacterium salinarium）中得到表征。当一滴氢气周围的气体。盐藻S9-P菌株在10%（v/v）O_2到100%N_2的突变条件下，细菌在适应并恢复随机游动之前，其逆转频率短暂增加。当气体恢复到10%O2时，细菌通过平稳游动约45 s作出反应。呼吸作用在H.盐藻的质子动力最高，而细菌视紫红质和盐视紫红质对质子动力没有贡献。营养缺陷型的蛋氨酸饥饿。salinaria基本上消除了降压趋氧反应。甲醇生产的甲基接受趋化蛋白的去甲基化是短暂增加在H。salinariumS 9-P通过氧浓度的逐步下降或逐步上升，如在H.盐疗室。紫杉醇阴性和甲基转移酶缺陷的突变体，H。salinariumstrain Pho 72对趋氧性或趋化性刺激的甲醇释放没有表现出变化。这是第一个报告的趋氧反应，是依赖于甲基接受趋化蛋白的甲基化。大肠杆菌和鼠伤寒沙门氏菌的嗜氧性不依赖于转导子甲基化。
The behavioural response to a gradient of oxygen (aerotaxis) has been characterized in the archaeon,Halobacterium salinarium. When the gas surrounding a drop ofH. salinariumstrain S9-P culture was changed abruptly from 10% (v/v) O2to 100% N2, the bacteria transiently increased the frequency of reversing before they adapted and resumed random swimming. When the gas was returned to 10% O2the bacteria responded by swimming smoothly for approximately 45 s. Aerotaxis was strongest when respiration inH. salinariumwas highest and when bacteriorhodopsin and halorhodopsin were not contributing to the proton motive force. Starvation for methionine of the auxotrophicH. salinariumessentially abolished the step-down aerotactic response. Methanol production from demethylation of methyl-accepting chemotaxis proteins was transiently increased inH. salinariumS9-P by a step down or step up in oxygen concentration, as observed in methylation-dependent chemotaxis inH. salinarium. The taxis-negative and methyltransferase-deficient mutant,H. salinariumstrain Pho72 did not exhibit changes in methanol release in response to aerotaxis or chemotaxis stimuli. This is the first report of an aerotactic response that is dependent on methylation of methyl-accepting chemotaxis proteins. Aerotaxis inEscherichia coliandSalmonella typhimuriumis independent of transducer methylation.