INHIBITION OF TRANSCRIPTION IN SOMATIC-CELLS BY MICROINJECTION OF ANTIBODIES TO CHROMOSOMAL-PROTEINS
INHIBITION OF TRANSCRIPTION IN SOMATIC-CELLS BY MICROINJECTION OF ANTIBODIES TO CHROMOSOMAL-PROTEINS
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DOI：
10.1073/pnas.80.22.6735
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
BUSTIN, M
中科院分区：
文献类型：
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作者：
EINCK, L;BUSTIN, M
将纯化抗体和抗体片段微注射到活的成纤维细胞中，检测确定的染色体蛋白的体内功能。在显微注射针对这些蛋白的荧光抗体后，通过研究[3H]尿苷在KD人成纤维细胞中的掺入，可以看到组蛋白和染色体高迁移性蛋白HMG-1、2和17在转录中的参与。微量注射对照抗体或微量注射不参与转录的染色体蛋白抗体后形成的免疫复合物不影响核尿苷的结合。注射抗组蛋白IgG、F（ab”）2或Fab和抗hmg -17 IgG可显著减少转录。这种减少与引入细胞的抗体量成正比。组蛋白和蛋白HMG-17存在于基因组的转录区域，RNA聚合酶沿着染色质纤维的传递被这些蛋白的抗体阻止。
The in vivo function of defined chromosomal proteins was examined by microinjecting purified antibody and antibody fragments into living fibroblasts. The involvement of histones and chromosomal high mobility group proteins HMG-1, 2 and 17 in transcription was visualized by studying the [3H]uridine incorporation in KD human fibroblasts after microinjection of fluoresceinated antibodies to these proteins. Nuclear uridine incorporation was not affected by microinjection of control antibodies or by the presence of immune complexes formed after microinjection of antibodies to chromosomal proteins that are not involved in transcription. Injection of anti-histone IgG, F(ab'')2, or Fab and anti-HMG-17 IgG causes a significant reduction in transcription. The reduction is proportional to the amount of antibody introduced into the cell. Histones and protein HMG-17 are present on transcribed regions of the genome and passage of RNA polymerase along the chromatin fiber is prevented by antibody to these proteins.