Urine lipoarabinomannan in HIV uninfected, smear negative, symptomatic TB patients: effective sample pretreatment for a sensitive immunoassay and mass spectrometry.
Urine lipoarabinomannan in HIV uninfected, smear negative, symptomatic TB patients: effective sample pretreatment for a sensitive immunoassay and mass spectrometry.
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HIV中的尿液脂肪脂瘤未感染，涂片阴性，有症状的结核病患者：敏感免疫测定和质谱法的有效样品预处理。
DOI：
10.1038/s41598-021-82445-4
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发表时间：
2021-02-03
影响因子：
4.6
通讯作者：
Chatterjee D
中科院分区：
文献类型：
--
作者：
Amin AG;De P;Graham B;Calderon RI;Franke MF;Chatterjee D
我们的研究试图确定尿脂阿拉伯甘露聚糖(LAM)是否可以在一个样本队列中得到验证，该样本队列主要由出现结核病症状的未感染HIV的人组成。我们评估了我们实验室开发的两种检测方法，并将它们用于来自艾滋病毒发病率较低的秘鲁利马的临床样本。用100g尿液μL对140例成人培养确诊结核病患者和20例症状阴性儿童进行了酶联免疫吸附试验(ELISA)检测。在病例中，光密度(OD450)值与较高的细菌负荷呈正相关。20个对照的值为负值(低于检测限值)。该分析正确地识别了所有样品(97%-100%的准确度可信区间)。为了对ELISA结果进行替代验证，我们使用抗体非依赖的化学分析方法分析了所有160个尿样。只有当LAM替代品-结核硬脂酸(TBSA)和d-阿拉伯糖(d-Ara)-被发现存在类似数量时，样本才被称为阳性。所有结核病病例，包括40例痰涂片呈阴性的病例，尿液中含有可检测到的LAM数量。所有对照组都没有检测到LAM的含量。我们的研究表明，尿液LAM检测在HIV未感染、涂阳肺结核患者中是可行的。
Our study sought to determine whether urine lipoarabinomannan (LAM) could be validated in a sample cohort that consisted mainly of HIV uninfected individuals that presented with tuberculosis symptoms. We evaluated two tests developed in our laboratory, and used them on clinical samples from Lima, Peru where incidence of HIV is low. ELISA analysis was performed on 160 samples (from 140 adult culture-confirmed TB cases and 20 symptomatic TB-negative child controls) using 100 μL of urine after pretreatment with Proteinase K. Two different mouse monoclonal antibodies-CS35 and CHCS9-08 were used individually for capture of urine LAM. Among cases, optical density (OD450) values had a positive association with higher bacillary loads. The 20 controls had negative values (below the limit of detection). The assay correctly identified all samples (97–100% accuracy confidence interval). For an alternate validation of the ELISA results, we analyzed all 160 urine samples using an antibody independent chemoanalytical approach. Samples were called positive only when LAM surrogates—tuberculostearic acid (TBSA) and d-arabinose (d-ara)—were found to be present in similar amounts. All TB cases, including the 40 with a negative sputum smear had LAM in detectable quantities in urine. None of the controls had detectable amounts of LAM. Our study shows that urinary LAM detection is feasible in HIV uninfected, smear negative TB patients.
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DOI：
10.1016/j.trstmh.2005.04.014
发表时间：
2005-12-01
影响因子：
2.2
作者：
Boehme, C;Molokova, E;Hoelscher, M
通讯作者：
Hoelscher, M
DOI：
10.1097/qai.0000000000000672
发表时间：
2015-08-01
期刊：
Journal of acquired immune deficiency syndromes (1999)
影响因子：
--
作者：
Lawn SD;Kerkhoff AD;Nicol MP;Meintjes G
通讯作者：
Meintjes G
影响因子：
3.7
作者：
Broger, Tobias;Tsionksy, Michael;Sigal, George B.
通讯作者：
Sigal, George B.
影响因子：
5.3
作者：
De, Prithwiraj;Shi, Libin;Chatterjee, Delphi
通讯作者：
Chatterjee, Delphi
DOI：
10.1093/trstmh/trw008
发表时间：
2016-03
影响因子：
2.2
作者：
Lawn SD;Gupta-Wright A
通讯作者：
Gupta-Wright A