Comparison of the coated-vesicle and synaptic-vesicle vacuolar (H+)-ATPases.
Comparison of the coated-vesicle and synaptic-vesicle vacuolar (H+)-ATPases.
复制标题
涂层囊泡和突触囊泡液泡 (H)-ATP 酶的比较。
DOI：
10.1111/j.1749-6632.1994.tb17270.x
复制
发表时间：
1994
影响因子：
5.2
通讯作者：
Forgac,M
中科院分区：
文献类型：
--
作者：
Rodman,J;Feng,Y;Myers,M;Zhang,J;Magner,R;Forgac,M
V-ATP酶是一类新型的ATP依赖性质子泵，负责真核细胞内区室的酸化。它们在受体介导的内吞作用、细胞内膜运输、大分子加工和降解以及偶联运输中起重要作用，并且在某些特化细胞类型的质膜中起作用。V-ATP酶是多亚基复合物，其被组织成负责ATP水解的外周V1复合物和负责质子转运的完整V0结构域。液泡酸化的调节对于其在膜运输和其他细胞过程中的作用至关重要。目前，我们正在研究几种机制的调节液泡酸化，包括位于催化位点的半胱氨酸残基之间的二硫键形成，控制组装的外围和积分域，和差异的V-ATP酶靶向不同的细胞内目的地使用它们的相互作用与细胞器特异性的适应复合物。
The V-ATPases are a novel class of ATP-dependent proton pumps responsible for acidification of intracellular compartments in eukaryotic cells. They play an important role in receptor-mediated endocytosis, intracellular membrane traffic, macromolecular processing and degradation and coupled transport, as well as functioning in the plasma membrane of certain specialized cell types. The V-ATPases are multisubunit complexes that are organized into a peripheral V1 complex responsible for ATP hydrolysis and an integral V0 domain responsible for proton translocation. Regulation of vacuolar acidification is critical to its role in membrane traffic and other cellular processes. We are currently investigating several mechanisms of regulation of vacuolar acidification, including disulfide bond formation between cysteine residues located at the catalytic site, control of assembly of the peripheral and integral domains, and differential targeting of V-ATPases to different intracellular destinations using their interaction with organelle-specific adaptin complexes.