The structure of adenovirion chromatin revealed by ultraviolet light-induced cross-linking.
The structure of adenovirion chromatin revealed by ultraviolet light-induced cross-linking.
复制标题
紫外光诱导交联揭示腺病毒染色质的结构。
DOI：
10.1016/0006-291x(81)91591-6
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
K. Hosokawa
中科院分区：
文献类型：
--
作者：
K. Sato;K. Hosokawa
Upon irradiating adenovirion by ultraviolet light, viral DNA and a part of viral protein cosedimented faster than free DNA when examined in the presence of 4 M guanidine HC1 in sucrose gradient, indicating that protein became tightly bound to DNA. By sodium dodecylsulfate polyacrylamide gel electrophoresis, it was verified that the protein is cross-linked covalently to DNA and that polypeptide VII is the only polypeptide species present. Based on the available data on the size of nuclease digested fragments, a model of adenovirion chromatin consisting of a nucleosome-like subunit of polypeptide VII-hexamer was constructed.
