Rupture of Lipid Membranes Induced by Amphiphilic Janus Nanoparticles
Rupture of Lipid Membranes Induced by Amphiphilic Janus Nanoparticles
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DOI：
10.1021/acsnano.8b00759
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发表时间：
2018-04-01
期刊：
影响因子：
17.1
通讯作者：
Yu, Yan
中科院分区：
文献类型：
--
作者：
Lee, Kwahun;Zhang, Liuyang;Yu, Yan
纳米颗粒的表面涂层决定了它们与生物膜的相互作用，但研究几乎仅限于具有均匀表面化学的纳米颗粒。尽管纳米颗粒越来越多地由复杂的表面化学组成以实现多功能性，但我们对异质表面涂层如何影响颗粒-生物膜相互作用的理解却远远落后。在这里，我们报告了这个问题的调查，在实验系统组成的两亲性的“双面”Janus纳米粒子和支持的脂质膜。我们发现，在皮摩尔浓度的两亲性Janus纳米粒子诱导缺陷的两性离子脂质双层。除了揭示粒子双层相互作用中的疏水性和电荷的各种影响，我们证明了Janus几何-疏水性和粒子表面电荷的空间分离导致纳米粒子更强烈地结合到双层，并比具有均匀混合表面功能的粒子更有效地诱导缺陷。我们将联合收割机实验与计算机模拟相结合，以进一步阐明两亲性Janus纳米颗粒如何提取脂质以破坏完整的脂质双层。这项研究提供了直接的证据，表明纳米颗粒表面功能的空间排列，而不仅仅是其整体表面化学，在决定它如何与生物膜相互作用方面起着至关重要的作用。
The surface coatings of nanoparticles determine their interaction with biomembranes, but studies have been limited almost exclusively to nanoparticles with a uniform surface chemistry. Although nanoparticles are increasingly made with complex surface chemistries to achieve multi-functionalities, our understanding of how a heterogeneous surface coating affects particle-biomembrane interaction has been lagging far behind. Here we report an investigation of this question in an experimental system consisting of amphiphilic "two-faced" Janus nanoparticles and supported lipid membranes. We show that amphiphilic Janus nanoparticles at picomolar concentrations induce defects in zwitterionic lipid bilayers. In addition to revealing the various effects of hydrophobicity and charge in particle bilayer interactions, we demonstrate that the Janus geometry-the spatial segregation of hydrophobicity and charges on particle surface causes nanoparticles to bind more strongly to bilayers and induce defects more effectively than particles with uniformly mixed surface functionalities. We combine experiments with computational simulation to further elucidate how amphiphilic Janus nanoparticles extract lipids to rupture intact lipid bilayers. This study provides direct evidence that the spatial arrangement of surface functionalities on a nanoparticle, rather than just its overall surface chemistry, plays a crucial role in determining how it interacts with biological membranes.