Nonalcoholic fatty liver disease is associated with lower hepatic and erythrocyte ratios of phosphatidylcholine to phosphatidylethanolamine
Nonalcoholic fatty liver disease is associated with lower hepatic and erythrocyte ratios of phosphatidylcholine to phosphatidylethanolamine
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DOI：
10.1139/apnm-2012-0261
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发表时间：
2013-03-01
期刊：
影响因子：
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通讯作者：
Allard, Johane P.
中科院分区：
文献类型：
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作者：
Arendt, Bianca M.;Ma, David W. L.;Allard, Johane P.
非酒精性脂肪性肝病（NAFLD）与肝脏脂质组成改变有关。动物研究表明，肝脏中磷脂酰胆碱（PC）与磷脂酰乙醇胺（PE）的比例有助于脂肪生成和炎症。这一比例可能受到PE N-甲基转移酶（PEMT）途径失调或低胆碱饮食的影响。肝脏的变化也可能影响循环中的脂质组成，例如红细胞中的脂质组成，因此可能可用作肝脏疾病的生物标志物。目前，没有研究评估NAFLD中肝脏和红细胞PC/PE比值。本研究旨在比较单纯性脂肪变性（SS）或非酒精性脂肪性肝炎（NASH）患者与健康对照者肝脏和红细胞中的PC/PE比值。采用质谱法测定28例经活检证实的NAFLD患者（14例SS，14例NASH）和9例健康活体肝供体（作为对照）的PC和PE。与对照组（3.14 [2.20-3.73]）相比，SS患者（中位数[范围]）（1.23 [0.27 -3.40]）和NASH患者（1.29 [0.77-3.22]）的肝脏PC/PE比值较低;均p < 0.001），但SS和NASH之间无差异。与对照组相比，SS患者的肝脏PC较低，PE较高，而NASH患者仅PE较高。与对照组相比，SS和NASH患者红细胞中的PC/PE比值也较低，因为两个患者组的PC均较低。红细胞PE各组间无差异。总之，NAFLD患者肝脏和红细胞中的PC/PE比低于健康对照，这可能在发病机制中发挥作用。其潜在机制需要进一步研究。
Nonalcoholic fatty liver disease (NAFLD) is associated with altered hepatic lipid composition. Animal studies suggest that the hepatic ratio of phosphatidylcholine (PC) to phosphatidylethanolamine (PE) contributes to steatogenesis and inflammation. This ratio may be influenced by dysregulation of the PE N-methyltransferase (PEMT) pathway or by a low-choline diet. Alterations in the liver may also influence lipid composition in circulation such as in erythrocytes, which therefore may have utility as a biomarker of hepatic disease. Currently, no study has assessed both liver and erythrocyte PC/PE ratios in NAFLD. The aim of this study was to compare the PC/PE ratio in the liver and erythrocytes of patients with simple steatosis (SS) or nonalcoholic steatohepatitis (NASH) with that of healthy controls. PC and PE were measured by mass spectrometry in 28 patients with biopsy-proven NAFLD (14 SS, 14 NASH) and 9 healthy living liver donors as controls. The hepatic PC/PE ratio was lower in SS patients (median [range]) (1.23 [0.27-3.40]) and NASH patients (1.29 [0.77-3.22]) compared with controls (3.14 [2.20-3.73]); both p < 0.001) but it was not different between SS and NASH. PC was lower and PE higher in the liver of SS patients compared with controls, whereas in NASH patients only PE was higher. The PC/PE ratio in erythrocytes was also lower in SS and NASH patients compared with controls because of lower PC in both patient groups. PE in erythrocytes was not different among the groups. In conclusion, NAFLD patients have a lower PC/PE ratio in the liver and erythrocytes than do healthy controls, which may play a role in the pathogenesis. The underlying mechanisms require further investigation.