Cell culture of individual Drosophila embryos. I. Development of wild-type cultures.
Cell culture of individual Drosophila embryos. I. Development of wild-type cultures.
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单个果蝇胚胎的细胞培养。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
J. H. Sang
中科院分区：
文献类型：
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作者：
D. P. Cross;J. H. Sang
本文介绍了一种从黑腹果蝇个体早期原肠中制备体外细胞培养物的新方法。在这些培养物中，几种可识别的细胞类型在24小时内分化（神经、肌肉、脂肪体、血细胞和分泌几丁质）；他们最初的外观和持续发展的一段时间内的描述。有人提出，该技术可用于分析胚胎致死性突变体的细胞发育异常。从致死胚胎中体外培养细胞被认为有两大作用：(1)测试单个细胞类型在相对不受其他细胞类型可能的有害相互作用的情况下的发育能力，并从垂死胚胎的系统中解放出来；(2)通过制备正常和突变胚胎的混合培养物，确定野生型细胞的存在对观察到的特定细胞类型异常的影响。
A new procedure is described for the preparation of in vitro cell cultures from individual early gastrulae of Drosophila melanogaster. In these cultures several identifiable cell types differentiate within 24 h (nerve, muscle, fat-body, haemocyte and chitin-secreting); their initial appearance and continuing development over a period of weeks is described. It is proposed that this technique may be used to analyse abnormalities of cellular development in embryonic lethal mutants. Culture in vitro of cells from lethal embryos is seen to have two broad roles: (1) to test the developmental capacity of individual cell types in a situation where they are relatively free from possible deleterious interactions with other cell types and are liberated from the system of the dying embryo, and (2) through the preparation of mixed cultures from normal and mutant embryos, to determine the influence of the presence of wild-type cells on observed abnormalities of a particular cell type.