Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres
Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres
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DOI：
10.1038/nature04277
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发表时间：
2006-01-12
期刊：
影响因子：
64.8
通讯作者：
Lanza, R
中科院分区：
文献类型：
--
作者：
Chung, Y;Klimanskaya, I;Lanza, R
对人类胚胎干细胞研究最基本的反对意见源于这样一个事实，即胚胎干细胞的衍生剥夺了胚胎进一步发育成完整人类的任何潜力(1，2)。ES细胞系通常从囊胚的内细胞团(3-5)分离出来，在少数情况下，从卵裂期胚胎(6-9)分离出来。到目前为止，还没有文献报道使用一种不需要破坏胚胎的方法来获得干细胞系。在这里，我们报告了一种建立ES细胞系的替代方法-使用与植入前遗传缺陷遗传诊断相似的单细胞胚胎活检技术(10)-不干扰胚胎的发育潜力。从单个卵裂球中分离出5个ES细胞系和7个滋养层干细胞系，这些细胞系在传代过程中保持了正常的核型和多能性标志，即TS细胞。ES细胞在体外和畸胎瘤中均可分化为三种胚层的衍生物，并表现出胚系传递。单个卵裂球活检的胚胎发育到足月，其发育能力没有下降。在不破坏体外胚胎的情况下产生人类胚胎干细胞的能力将减少或消除许多人的伦理担忧。
The most basic objection to human embryonic stem (ES) cell research is rooted in the fact that ES cell derivation deprives embryos of any further potential to develop into a complete human being(1,2). ES cell lines are conventionally isolated from the inner cell mass of blastocysts(3-5) and, in a few instances, from cleavage stage embryos(6-9). So far, there have been no reports in the literature of stem cell lines derived using an approach that does not require embryo destruction. Here we report an alternative method of establishing ES cell lines - using a technique of single-cell embryo biopsy similar to that used in pre-implantation genetic diagnosis of genetic defects(10) - that does not interfere with the developmental potential of embryos. Five putative ES and seven trophoblast stem (TS) cell lines were produced from single blastomeres, which maintained normal karyotype and markers of pluripotency or TS cells for up to more than 50 passages. The ES cells differentiated into derivatives of all three germ layers in vitro and in teratomas, and showed germ line transmission. Single-blastomere-biopsied embryos developed to term without a reduction in their developmental capacity. The ability to generate human ES cells without the destruction of ex utero embryos would reduce or eliminate the ethical concerns of many.