Effects of ovariectomy on the inflammatory responses of female rats to the central injection of lipopolysaccharide
Effects of ovariectomy on the inflammatory responses of female rats to the central injection of lipopolysaccharide
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DOI：
10.1016/j.jneuroim.2014.09.017
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发表时间：
2014-12-15
影响因子：
3.3
通讯作者：
Irahara, Minoru
中科院分区：
文献类型：
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作者：
Iwasa, Takeshi;Matsuzaki, Toshiya;Irahara, Minoru
据报道，肥胖会以特定部位的方式导致更明显的炎症反应。正如在其他肥胖动物模型中所看到的那样，去卵巢的啮齿动物对全身注射脂多糖（LPS）表现出肥胖和加剧的发烧和厌食反应。此外，它们在一些中枢和外周组织中也显示出促炎细胞因子的表达增加。有趣的是，在下丘脑中观察到的改变比在其他周围组织中观察到的更明显。本研究采用中央LPS注射法评估卵巢切除术对下丘脑炎症反应的影响。将LPS注射到去卵巢和性腺完整的雌性大鼠脑室内，比较两组大鼠的免疫应答。切除卵巢的大鼠比性腺完整的大鼠体重更重。此外，切除卵巢的大鼠比性腺完整的大鼠表现出更强的发热反应。体外注射LPS后，去卵巢大鼠下丘脑白细胞介素(IL)-1 β、IL-6和环氧化酶2 mRNA水平显著高于性腺完整大鼠。研究了补充雌二醇对大鼠免疫反应的影响。然而，在体外注射LPS后，补充雌二醇的去卵巢大鼠的发热反应和下丘脑IL-1 β、IL-6和tnf - α mRNA水平以及体重匹配的油给药（对照）大鼠没有差异。这些结果表明，去卵巢大鼠下丘脑对LPS的敏感性增加，这种变化是由性腺类固醇缺乏的间接影响引起的。正如在其他肥胖动物模型中所看到的，卵巢切除引起的肥胖可能在卵巢切除大鼠中观察到的炎症反应加剧中起重要作用。(C) 2014 Elsevier B.V.版权所有
It has been reported that obesity leads to more marked inflammatory responses in a site-specific manner. As has been seen in other animal models of obesity, ovariectomized rodents exhibit obesity and exacerbated fever and anorectic responses to the systemic injection of lipopolysaccharide (LPS). Furthermore, they also display increased pro-inflammatory cytokine expression in several central and peripheral tissues. Interestingly, the alterations observed in the hypothalamus are more marked than those seen in other peripheral tissues. In this study, the effects of ovariectomy on hypothalamic inflammatory responses were evaluated using the central LPS injection method. LPS was intracerebroventricularly (i.c.v.) injected into ovariectomized and gonadally intact female rats, and the immune responses of the two groups were compared. The ovariectomized rats exhibited heavier body weights than the gonadally intact rats. In addition, the ovariectomized rats displayed stronger febrile responses than the gonadally intact rats. After the i.c.v. injection of LPS, the hypothalamic interleukin (IL)-1 beta, IL-6, and cyclooxygenase 2 mRNA levels of the ovariectomized rats were significantly higher than those of the gonadally intact rats. The effects of estradiol supplementation on the rats' immune responses were also examined. However, the febrile responses and hypothalamic IL-1 beta, IL-6, and TNF-alpha mRNA levels of estradiol-supplemented ovariectomized rats and body weight matched oil-administered (control) rats did not differ after the i.c.v. injection of LPS. These results indicate that hypothalamic sensitivity to LPS is increased in ovariectomized rats and that this change is induced by the indirect effects of gonadal steroid deficiency. As is seen in other obese animal models, ovariectomy-induced obesity might play important roles in the exacerbated inflammatory responses observed in ovariectomized rats. (C) 2014 Elsevier B.V. All rights reserved.