Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans
Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans
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DOI：
10.1111/j.1399-302x.2008.00480.x
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发表时间：
2009-04-01
影响因子：
--
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
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作者：
Inagaki, S.;Matsumoto-Nakano, M.;Ooshima, T.
重组酶A （RecA）在肺炎链球菌的质粒和染色体DNA转化中都是必不可少的，并且被认为与变形链球菌的sos应答有关。本研究通过插入失活S. mutans菌株MT8148中编码recA蛋白的recA基因，构建了recA缺陷突变株（RAD），并对其耐酸和生物膜形成的生物学功能进行了研究。与野生型菌株相比，RAD的耐酸性降低，产生的生物膜密度降低。此外，共聚焦显微镜观察表明，与菌株MT8148产生的生物膜相比，RAD产生的生物膜由活力明显降低的细胞组成。这些结果表明RecA与生物膜的形成有关。
Recombinase A (RecA) is essential for the transformation of both plasmid and chromosomal DNA in Streptococcus pneumoniae and is considered to be related to the SOS-response in Streptococcus mutans.In the present study, a RecA-deficient mutant strain (RAD) was constructed by insertional inactivation of the recA gene encoding the RecA protein in strain MT8148 of S. mutans, after which the biological functions of acid tolerance and biofilm formation were investigated.RAD showed reduced acid tolerance and produced lower density biofilm compared with the wild-type strain. In addition, confocal microscopic observation indicated that the biofilm produced by RAD was composed of cells with significantly lower viability compared with that produced by strain MT8148.These results suggest that RecA has a relationship with biofilm formation.