In Situ, Label-Free DNA Detection Using Organic Transistor Sensors
In Situ, Label-Free DNA Detection Using Organic Transistor Sensors
复制标题
DOI：
10.1002/adma.201000790
复制
发表时间：
2010-10-25
影响因子：
29.4
通讯作者：
Bao, Zhenan
中科院分区：
文献类型：
--
作者：
Khan, Hadayat Ullah;Roberts, Mark E.;Bao, Zhenan
快速和高灵敏度的PNA-DNA杂交检测在从基因分型到分子诊断的广泛应用中引起了极大的关注。基于微阵列和实时PCR的常规光学检测系统涉及昂贵的检测方案，通常需要荧光染料和光源/检测器;然而，该方法已成为用于定量表面固定化探针和荧光团标记的DNA靶之间的杂交程度的标准技术。化学检测研究的最新进展，部分得益于有机电子学的巨大进步，已经显示出对当前光学检测系统的可行的、低成本的替代方案的巨大希望。[3，4]有机晶体管技术在化学传感器中的应用尤其令人鼓舞。这种简单的平台允许制造低成本，大面积和灵活的设备，具有空气稳定性，低功耗，生物相容性和容易的表面改性，用于检测各种分析物。[5，6]存在许多使用OTFT平台检测分析物蒸汽的示例，许多报告都涉及通过使用指纹响应[7，8]或通过在功能OTFT上结合选择性检测层来识别特定分析物的能力。[4，9]已经证明了水系统中化学检测的几个例子;然而，这些设备对特定的分析物没有选择性。[3，10] OTFT的选择性原位检测需要一种通用方法，用于将各种选择性分子探针固定在活性转运通道附近。
Rapid and highly sensitive PNA-DNA hybridization assays have attracted enormous attention for a wide variety of applications ranging from genotyping to molecular diagnosis.[1, 2] Conventional optical detection systems based on microarrays and real-time PCR involve expensive detection protocols, typically requiring a fluorescent dye and optical sources/detectors; however, this method has become the standard technique for quantifying the extent of hybridization between surface immobilized probes and fluorophore-labeled DNA targets. Recent advances in chemical detection research, in part benefiting from the overwhelming progress made in organic electronics, have shown great promise for a viable, low-cost alternative to current optical detection systems.[3, 4] The utilization of organic transistor technology in chemical sensors is particularly encouraging. This simple platform allows for the fabrication of low-cost, large-area, and flexible devices with air stability, low-power consumption, biocompatibility, and facile surface modification for the detection of a wide range of analyte species.[5, 6] Many examples exist for the detection of analyte vapors using an OTFT platform, with numerous reports addressing the ability to identify particular analytes either through the use of a fingerprint response [7, 8] or by incorporating selective detection layers on functional OTFTs.[4, 9] Few examples of chemical detection in aqueous systems have been demonstrated; however, these devices were not selective toward a particular analyte.[3, 10] Selective in situ detection with OTFTs requires a versatile method for the immobilization of various selective molecular probes within proximity to the active transport channel.