Separation and analysis of ginsenoside Rg1: an experiment for the separation and analysis of natural products
Separation and analysis of ginsenoside Rg1: an experiment for the separation and analysis of natural products
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人参皂苷Rg1的分离分析：天然产物的分离分析实验
DOI：
10.1021/acs.jchemed.9b00541
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发表时间：
2020
影响因子：
3
通讯作者：
Chen Bo
中科院分区：
文献类型：
--
作者：
Yang Rong;Hu Yang Y.;Huang Si;Fang Zheng F.;Ma Ming;Chen Bo
介绍了一种针对制药工程本科三年级学生的综合性实验。学生此前已完成分析化学、天然产物化学、生物分离工程等课程，具备纯化、定量分析、填充柱色谱、高效液相色谱、薄层色谱等背景知识。学生被分成四到六人一组，每个学生组独立完成整个实验。以人参茎叶提取物为原料。学生采用高效液相色谱法分别采用面积归一化法和绝对含量法分析原料和纯化产品中Rg1的含量；然后学生们计算产量。硅胶柱层析时，采用薄层色谱快速监测层析组分，准确收集含有Rg1的组分。采用高效液相色谱法外标法对所得Rg1进行定量分析。通过本实验，学生可以从分析水平到准备水平，从快速定性监测到定量分析，获得对分离技术的概念理解，以及实际操作技能和理解。
A comprehensive experiment for third-year undergraduate students in pharmaceutical engineering is described. Students had previously completed courses in analytical chemistry, chemistry of natural products, and bioseparation engineering and had background knowledge in purification, quantitative analysis, packed column chromatography, high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and so on. Students were arranged into groups of four to six, and each student group completed the whole experiment independently. Extracts of Panax ginseng stems and leaves were used as raw materials. Students used HPLC to analyze the contents of Rg1 in the raw materials and the purified products by the area normalization method and the absolute content method, respectively; students then calculated the yield. During silica gel column chromatography, TLC was used to quickly monitor the chromatographic fractions to accurately collect fractions containing Rg1. The pure Rg1 obtained was quantitatively analyzed by HPLC with an external standard method. Conducting this experiment, students can gain conceptual understanding as well as practical skills and understanding of separation techniques from the analytical level to the preparative level and from rapid qualitative monitoring to quantitative analysis.