C/EBP β mRNA expression is upregulated and positively correlated with the expression of TNIP1/TNFAIP3 in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
C/EBP β mRNA expression is upregulated and positively correlated with the expression of TNIP1/TNFAIP3 in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
复制标题
系统性红斑狼疮患者外周血单个核细胞中 C/EBP β mRNA 表达上调并与 TNIP1/TNFAIP3 表达呈正相关
DOI：
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发表时间：
2016
影响因子：
2.7
通讯作者：
张东梅
中科院分区：
文献类型：
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作者：
钱添;陈艳;史晓尉;李健;郝飞;张东梅
CCAAT/增强子结合蛋白β（C/EBP β）在多种信号通路中发挥重要作用。系统性红斑狼疮（SLE）患者C/EBP β的大多数调节因子和靶基因产物（包括肿瘤坏死因子α诱导蛋白3（TNFAIP 3）和TNFAIP 3相互作用蛋白1（TNIP 1））的表达上调。本研究的目的是探讨C/EBP β表达是否与SLE发病机制相关以及与TNIP 1和TNFAIP 3表达的相关性。应用定量逆转录-聚合酶链反应（RT-PCR）检测20例SLE患者和20例健康对照者外周血单个核细胞（PBMC）中C/EBP β、TNIP 1和TNFAIP 3 mRNA的表达。采用斯皮尔曼秩和检验（Spearman's rank test）分析SLE患者PBMC中C/EBP β表达与疾病活动性的相关性，以及C/EBP β表达与TNIP 1/TNFAIP 3表达的相关性。SLE患者C/EBP β mRNA表达较正常对照组明显增高。C/EBP β表达与SLE疾病活动指数呈正相关，与血清补体C3、C4水平呈负相关。此外，抗核抗体、抗Smith抗体和抗nRNP抗体阳性的SLE患者PBMC中C/EBP β mRNA的表达较抗体阴性的SLE患者明显增加。SLE患者C/EBP β mRNA表达水平与TNIP1、TNFAIP3表达水平呈正相关。本研究结果提示，C/EBP β在PBMCs中的高表达以及C/EBP β与TNIP1/TNFAIP3的相互作用可能参与了SLE的发病过程。
CCAAT/enhancer-binding protein β (C/EBP β) has important roles in numerous signaling pathways. The expres- sion of the majority of regulators and target gene products of C/EBP β, including tumor necrosis factor α-induced protein 3 (TNFAIP3) and TNFAIP3-interacting protein 1 (TNIP1), are upregulated in patients with systemic lupus erythematosus (SLE). The aim of the present study was to investigate whether C/EBP β expression is associated with SLE pathogenesis and correlates with TNIP1 and TNFAIP3 expression. Quantitative reverse transcription-polymerase chain reaction analysis was used to assess the expression of C/EBP β, TNIP1, and TNFAIP3 mRNA in peripheral blood mononuclear cells (PBMC) from 20 patients with SLE and 20 healthy controls. Spearman's rank test was used to determine the correlation between C/EBP β expression and SLE disease activity, and that between C/EBP β expression and TNIP1/TNFAIP3 expression in PBMCs from patients with SLE. C/EBP β mRNA expression was markedly increased in patients with SLE compared with healthy controls. The expression of C/EBP β was positively correlated with the SLE disease activity index and negatively correlated with the serum level of complement components C3 and C4. In addition, C/EBP β mRNA expression was increased in PBMCs from SLE patients that were positive for antinuclear, anti-Smith and anti-nRNP antibodies, compared with the antibody negative SLE patients. Furthermore, the mRNA expression levels of C/EBP β in patients with SLE was positively correlated with TNIP1 and TNFAIP3 expression. The results of the current study suggest that the increased expression of C/EBP β in PBMCs and the interaction between C/EBP β and TNIP1/TNFAIP3 may be involved in the patho- genesis of SLE.