Ultrastructure of the mammillotegmental projections to the ventral tegmental nucleus of gudden in the rat
Ultrastructure of the mammillotegmental projections to the ventral tegmental nucleus of gudden in the rat
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大鼠乳头被盖投射到腹侧被盖核的超微结构
DOI：
10.1002/cne.902930309
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发表时间：
1990
影响因子：
2.5
通讯作者：
K. Zyo
中科院分区：
文献类型：
--
作者：
T. Hayakawa;K. Zyo
本研究通过顺行转运与麦芽凝集素 (WGA-HRP) 结合的辣根过氧化物酶并用四甲基联苯胺可视化，检查了大鼠 Gudden (TV) 腹侧被盖核内内侧乳头核轴突的终止模式。 TV 的神经纤维包含三类轴突末梢，即包含圆形、扁平和多形性突触小泡的末梢。这些类型分别占所有正常轴突末端的 55.6%、26.1% 和 18.3%。将 WGA-HRP 注射到内侧乳头核中，可以对电视内顺行标记的终端进行超微结构识别。超过 80% 的标记末端含有圆形突触小泡并形成不对称的突触接触，而约 16% 的标记末端含有具有对称突触接触的扁平突触小泡。有一些带有多形性囊泡的标记末端，并且只有少数轴体末端。几乎所有带标签的端子都很小，直径小于 1.5 μm。与正常和标记的圆形囊泡末端的分布相比，中间树突（直径1-2μm）上的圆形囊泡标记末端的百分比增加，而远端树突（直径小于1μm）的标记末端百分比减少。顺行标记的轴突末端经常接触逆行标记的树突。
This study examines the termination pattern of axons from the medial mammillary nucleus within the ventral tegmental nucleus of Gudden (TV) in rats by using anterograde transport of horseradish peroxidase conjugated with wheat germ agglutinin (WGA‐HRP) and visualized with tetramethylbenzidine. The neuropil of TV contains three classes of axodendritic terminals, that is, terminals containing round, flat, and pleomorphic synaptic vesicles. These types make up 55.6%, 26.1%, and 18.3%, respectively, of all normal axodendritic terminals. Injection of WGA‐HRP into the medial mammillary nucleus permits ultrastructural recognition of anterogradely labeled terminals within the TV. More than 80% of the labeled terminals contain round synaptic vesicles and form asymmetric synaptic contacts, whereas about 16% contain flat synaptic vesicles with symmetric synaptic contacts. There are a few labeled terminals with pleomorphic vesicles and only a few axosomatic terminals. Almost all labeled terminals are small, having diameters of less than 1.5 μm. Compared with the distributions of normal and labeled terminals with round vesicles, there is an increase of the percentage of labeled terminals with round vesicles on the intermediate dendrites (1‐2 μm diameter) and a decrease on the distal dendrites (less than 1 μm diameter). Anterogradely labeled axon terminals often contact retrogradely labeled dendrites.