Assignment of cytosine-5 DNA methyltransferases Dnmt3a and Dnmt3b to mouse chromosome bands 12A2-A3 and 2H1 by in situ hybridization
Assignment of cytosine-5 DNA methyltransferases Dnmt3a and Dnmt3b to mouse chromosome bands 12A2-A3 and 2H1 by in situ hybridization
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DOI：
10.1159/000015331
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发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Li, E
中科院分区：
文献类型：
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作者：
Okano, M;Takebayashi, S;Li, E
本文用原位杂交法对小鼠染色体12A2-A3和2H1带进行了胞嘧啶-5DNA甲基转移酶DNMT3A和DNMT3B的定位。C-5位胞嘧啶甲基化是脊椎动物DNA修饰的一种主要形式，在基因表达和发育调控中发挥重要作用。表达模式和生化分析表明，DNMT3A和DNMT3B可能负责从头甲基化，这是在发育过程中建立DNA甲基化模式的关键过程。
This article presents localization of cytosine-5 DNA methyltransferases Dnmt3a and Dnmt3bto mouse chromosome bands 12A2-A3 and 2H1 by in situ hybridization. The methylation of cytosine at the C-5 position is a major form of DNA modification in vertebrates and plays important roles in regulation of gene expression and development. Expression pattern and biochemical analysis suggest that Dnmt3a and Dnmt3b are probably responsible for de novo methylation, a key process by which DNA methylation patterns are established during development.