Surface Modification of Electrospun Polyvinylidene Fluoride Nanofiber Membrane by Plasma Treatment for Protein Detection
Surface Modification of Electrospun Polyvinylidene Fluoride Nanofiber Membrane by Plasma Treatment for Protein Detection
复制标题
等离子体处理电纺聚偏氟乙烯纳米纤维膜表面改性用于蛋白质检测
DOI：
10.1166/jnn.2013.6925
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发表时间：
2013
影响因子：
--
通讯作者：
J.-K. Kook,
中科院分区：
文献类型：
--
作者：
E. Cho;C. Kim;J. Y. Park;C. H. Hwang;J. H. Kim;Y. A. Kim;M. Endo;D. R. Chang;J.-K. Kook,
静电纺聚偏氟乙烯（PVDF）纳米纤维膜具有三维开孔通道，在Western blot中具有良好的应用潜力。在本研究中，我们对静电纺PVDF纳米纤维膜进行氩等离子体处理，以提高其表面亲水性和检测灵敏度。结果表明，在不需要甲醇预湿步骤的情况下，Ar等离子体处理的PVDF纳米纤维膜的检测灵敏度随等离子体处理时间的增加而增加。这表明Ar等离子体处理的PVDF纳米纤维膜可用于高灵敏度的Western blot，无需甲醇预湿步骤。
Electrospun polyvinylidene fluoride (PVDF) nanofiber membranes have 3-dementional (3-D) open pore channel and hence have excellent application potential in Western blot. In this study we have modified electrospun PVDF nanofiber membrane by argon (Ar) plasma treatment to improve the surface hydrophilic and detection sensitivity. The results showed that the detection sensitivity of the Ar plasma-treated PVDF nanofiber membrane increased with increasing plasma treatment time without the need for a methanol pre-wet step. This suggests that the Ar plasma treated PVDF nanofiber membrane can be useful in Western blot with high sensitivity and without methanol prewet step.