The Standard FISH Procedure
The Standard FISH Procedure
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DOI：
10.1007/978-3-662-52959-1_9
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Rittscher, Katharina
中科院分区：
文献类型：
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作者：
Liehr, Thomas;Kreskowski, Katharina;Rittscher, Katharina
分子细胞遗传学最初包括两种基本方法：荧光原位杂交（FISH）和引物原位杂交（PRINS）。FISH技术是目前分子细胞遗传学研究和常规分子细胞遗传学领域的一种常规方法。这里描述了如何使用商业和/或自制DNA探针进行FISH的基本协议。关于组织切片上的FISH方案，请参见托马斯·利赫尔的章节“存档福尔马林固定/石蜡包埋或冷冻固定组织的表征，包括细胞核提取。“除了直接标记的探针外，还可以使用间接标记的探针，这里概述了两种变体。
Molecular cytogenetics originally comprised of two basic approaches: fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS). Nowadays FISH is the one routine approach still used in research and routine molecular cytogenetics field. Here the basic protocol in how to do FISH using commercial and/or homemade DNA probes is described. For a protocol for FISH on tissue sections, please refer to chapter by Thomas Liehr "Characterization of Archived Formalin-Fixed/Paraffin-Embed ded or Cryofixed Tissue, Including Nucleus Extraction." Besides direct-labeled probes, also indirect-labeled probes can be used, and both variants are outlined here.