Reagentless Bidirectional Lateral Flow Bioactive Paper Sensors for Detection of Pesticides in Beverage and Food Samples
Reagentless Bidirectional Lateral Flow Bioactive Paper Sensors for Detection of Pesticides in Beverage and Food Samples
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DOI：
10.1021/ac901714h
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发表时间：
2009-11-01
影响因子：
7.4
通讯作者：
Brennan, John D.
中科院分区：
文献类型：
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作者：
Hossain, S. M. Zakir;Luckham, Roger E.;Brennan, John D.
研制了一种用于检测乙酰胆碱酯酶（AChE）抑制剂（包括有机磷农药）的无试剂生物活性纸基固相生物传感器。测定条由纸支架（1 x 10 cm）组成，在两个不同的区域（例如，传感区和底物区），使用生物相容性溶胶凝胶衍生的二氧化硅墨水将AChE和显色底物吲哚乙酸酯（IPA）包裹在纸支架上。测定方案包括首先通过含农药溶液的横向流动将样品引入感应区。经过一段孵育期后，将纸张载体的另一端放入蒸馏去离子水（ddH(2)O）中，允许相反方向的横向流动将纸质IPA移动到感应区域，以启动酶催化底物的水解，导致黄色变为蓝色。改进后的传感器能够在不使用任何外部试剂的情况下检测农药，具有极好的检出限（恶威1 nM，卡威10 nM，对氧磷1 nM，马拉硫磷10 nM）和快速响应时间（5 min左右）。该传感器条在检测加标牛奶和苹果汁样品中的农药时显示基质效应可以忽略不计。生物活性纸基检测食品中农药残留的方法与传统的质谱分析方法一致。因此，生物活性纸试验应适用于环境和食品样品中痕量有机磷和氨基甲酸酯农药的快速筛选。
A reagentless bioactive paper-based solid-phase biosensor was developed for detection of acetylcholinesterase (AChE) inhibitors, including organophosphate pesticides. The assay strip is composed of a paper support (1 x 10 cm), onto which AChE and a chromogenic substrate, indophenyl acetate (IPA), were entrapped using biocompatible sol gel derived silica inks in two different zones (e.g., sensing and substrate zones). The assay protocol involves first introducing the sample to the sensing zone via lateral flow of a pesticide-containing solution. Following an incubation period, the opposite end of the paper support is placed into distilled deionized water (ddH(2)O) to allow lateral flow in the opposite direction to move paper-bound IPA to the sensing area to initiate enzyme catalyzed hydrolysis of the substrate, causing a yellow-to-blue color change. The modified sensor is able to detect pesticides without the use of any external reagents with excellent detection limits (bendiocarb 1 nM; carbatyl similar to 10 nM; paraoxon similar to 1 nM; malathion similar to 10 nM) and rapid response times (similar to 5 min). The sensor strip showed negligible matrix effects in detection of pesticides in spiked milk and apple juice samples. Bioactive paper-based assays on pesticide residues collected from food samples showed good agreement with a conventional mass spectrometric assay method. The bioactive paper assay should, therefore, be suitable for rapid screening of trace levels of organophosphate and carbamate pesticides in environmental and food samples.