Localization of core spindle pole body (SPB) components during SPB duplication in Saccharomyces cerevisiae.
Localization of core spindle pole body (SPB) components during SPB duplication in Saccharomyces cerevisiae.
复制标题
酿酒酵母中SPB重复期间，核心主体（SPB）组件的定位。
DOI：
10.1083/jcb.145.4.809
复制
发表时间：
1999-05-17
影响因子：
7.8
通讯作者：
Kilmartin, J V
中科院分区：
文献类型：
--
作者：
Adams, I R;Kilmartin, J V
我们用电子显微镜观察了酿酒酵母纺锤体极体（SPB）复制的过程，发现了几个阶段。这些包括大会，可能从卫星，一个大的斑块状结构，复制斑块，在细胞质面的半桥和它的插入到核膜。我们分析了这些结构的形成中的主要SPB组分的作用，通过质谱从SPB核心馏分中识别它们。制备了两种组分Spc29p和Nud1p的温度敏感突变体，以部分确定其功能。SPB复制，卫星和复制斑块的中间体的组成，通过免疫电镜检查。两者都含有细胞质SPB成分，表明当卫星形成时，在前一个细胞周期结束时已经部分实现了复制。我们表明，通过过度表达的SPB组件的卫星的结构可以改变和SPB复制抑制破坏附着的斑块样中间的半桥。我们提出了一个模型SPB复制SPB组件的绑定到桥结构的任何一端，确保两个单独的SPB。
We have examined the process of spindle pole body (SPB) duplication in Saccharomyces cerevisiae by electron microscopy and found several stages. These include the assembly, probably from the satellite, of a large plaque-like structure, the duplication plaque, on the cytoplasmic face of the half-bridge and its insertion into the nuclear envelope. We analyzed the role of the main SPB components in the formation of these structures by identifying them from an SPB core fraction by mass spectrometry. Temperature-sensitive mutants for two of the components, Spc29p and Nud1p, were prepared to partly define their function. The composition of two of the intermediates in SPB duplication, the satellite and the duplication plaque, was examined by immunoelectron microscopy. Both contain cytoplasmic SPB components showing that duplication has already been partly achieved by the end of the preceding cell cycle when the satellite is formed. We show that by overexpression of SPB components the structure of the satellite can be changed and SPB duplication inhibited by disrupting the attachment of the plaque-like intermediate to the half-bridge. We present a model for SPB duplication where binding of SPB components to either end of the bridge structure ensures two separate SPBs.