Selective proliferation of rat hepatocyte progenitor cells in serum-free culture
Selective proliferation of rat hepatocyte progenitor cells in serum-free culture
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DOI：
10.1038/nprot.2007.118
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Mitaka, Toshihiro
中科院分区：
文献类型：
--
作者：
Chen, Qijie;Kon, Junko;Mitaka, Toshihiro
该方案详细描述了使用透明质酸（HA）包被的培养皿和无血清培养基获得选择性增殖的肝细胞祖细胞的方法。小肝细胞（SH）是成人肝脏的肝细胞祖细胞，具有多种肝脏功能。当大鼠SH开始增殖时，CD 44特异性表达。为了确定SH的纯化，使用CD 44和细胞角蛋白8作为标记蛋白。SH在HA包被的培养皿上的生长比在其他细胞外基质包被的培养皿上的生长更快。使用DMEM/ F12培养基和HA包被的培养皿允许在培养中选择性地增殖SH。SH的纯化在第10天约为85%。
This protocol details a method of obtaining selectively proliferated hepatocyte progenitor cells using hyaluronic acid ( HA)-coated dishes and serum-free medium. A small hepatocyte ( SH) is a hepatocyte progenitor cell of adult livers and has many hepatic functions. When the rat SH begins to proliferate, CD44 is specifically expressed. To define the purification of SH, CD44 and cytokeratin 8 are used as marker proteins. The growth of SHs is faster on HA-coated dishes than on other extracellular matrix -coated ones. The use of both DMEM/ F12 medium and HA-coated dishes allows the selective proliferation of SHs in culture. The purification of SHs is approximately 85% at day 10.