Determination of 9, 10-phenanthrenequinone in airborne particulates by high-performance liquid chromatography with post-column fluorescence derivatization using 2-aminothiophenol
Determination of 9, 10-phenanthrenequinone in airborne particulates by high-performance liquid chromatography with post-column fluorescence derivatization using 2-aminothiophenol
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DOI：
10.1016/j.talanta.2010.03.053
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发表时间：
2010-06-15
期刊：
影响因子：
6.1
通讯作者：
Kuroda, Naotaka
中科院分区：
文献类型：
--
作者：
Kishikawa, Naoya;Nakashima, Hajime;Kuroda, Naotaka
9，10-菲醌（PQ）是一种有害的环境污染物，在大气环境中存在较多。研究发现，在酸性条件下，PQ与2-氨基苯硫酚反应生成荧光衍生物，在510 nm处发出绿色荧光。基于此反应，建立了一种简便、快速测定PQ的高效液相色谱柱后衍生化荧光检测方法。该方法在24 min内可检出PQ，检出限为67 fmol/injection（S/N = 3）。所提出的方法能够通过直接注射空气颗粒物的提取物来确定大气PQ浓度Crown Copyright（C）2010由Elsevier B V出版保留所有权利
9, 10-Phenanthrenequinone (PQ) is regarded as a harmful environmental pollutant and its presence has been reported in atmospheric environment. The measurement of PQ in environment should be necessary to evaluate the influence of PQ on human health We found that PQ reacted with 2-aminothiophenol under acidic condition to form fluorescent derivative which emits green fluorescence at 510 nm. Based on this reaction, a simple and rapid determination method for PQ was developed by HPLC with post-column derivatization and fluorescence detection By the proposed HPLC system, PQ was detected at 24 min and the detection limit was 67 fmol/injection (S/N = 3). The proposed method was able to determine the atmospheric PQ concentrations by the direct injection of extract from airborne particulates Crown Copyright (C) 2010 Published by Elsevier B V All rights reserved