1,25-dihydroxyvitamin D3 stimulates cyclic vitamin D receptor/retinoid X receptor DNA-binding, co-activator recruitment, and histone acetylation in intact osteoblasts
1,25-dihydroxyvitamin D3 stimulates cyclic vitamin D receptor/retinoid X receptor DNA-binding, co-activator recruitment, and histone acetylation in intact osteoblasts
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DOI：
10.1359/jbmr.041112
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发表时间：
2005-02-01
影响因子：
6.2
通讯作者：
Pike, JW
中科院分区：
文献类型：
--
作者：
Kim, S;Shevde, NK;Pike, JW
1,25(OH)(2)D-3通过VDR诱导基因表达。我们使用染色质免疫沉淀技术来探索完整成骨细胞中25-羟基维生素d -3-24-羟化酶（Cyp24）和开放蛋白基因启动子在1,25(OH)(2) d -3诱导的过程。我们的研究表明，1,25(OH)(2) d -3诱导的转激活是一个动态过程，涉及启动子特异性定位VDR和RXR，组蛋白乙酰转移酶复合物的募集，以及在Cyp24基因的情况下，组蛋白4的修饰。
1,25(OH)(2)D-3 induces gene expression through the VDR. We used chromatin immunoprecipitation techniques to explore this 1,25(OH)(2)D-3-induced process on the 25-hydroxyvitamin D-3-24-hydroxylase (Cyp24) and Opn gene promoters in intact osteoblasts. Our studies show that 1,25(OH)(2)D-3-induced transactivation is a dynamic process that involves promoter-specific localization of VDR and RXR, recruitment of histone acetyltransferase complexes, and in the case of the Cyp24 gene, modification of histone 4.