Antigenicity of Neisseria gonorrhoeae outer membrane protein(s) III detected by immunoprecipitation and Western blot transfer with a monoclonal antibody
Antigenicity of Neisseria gonorrhoeae outer membrane protein(s) III detected by immunoprecipitation and Western blot transfer with a monoclonal antibody
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使用单克隆抗体通过免疫沉淀和蛋白质印迹转移检测淋病奈瑟菌外膜蛋白 III 的抗原性
DOI：
10.1128/iai.38.2.668-672.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
Milton R. Tam
中科院分区：
文献类型：
--
作者：
John;Swanson;Leonard W. Mayer;Milton R. Tam
通过使用单克隆抗体 (2E6) 在蛋白质印迹转移和免疫沉淀反应中研究了淋球菌外膜 III 的一些特性。通过蛋白质印迹，2E6 单克隆抗体具有单一抗原靶标：外膜蛋白 III。检查的所有三种淋球菌菌株均表现出与该单克隆蛋白 III 相同的反应性。当 125I 标记的淋球菌与单克隆抗体 2E6 一起孵育，在 Zwittergent 中裂解，并用蛋白 A-Sepharose 免疫沉淀抗体-抗原复合物时，无论使用何种淋球菌菌株，外膜蛋白 I 和 III 都存在于免疫沉淀物中。淋球菌接触碘和 2-巯基乙醇会影响十二烷基硫酸钠聚丙烯酰胺凝胶电泳中蛋白质 III 的电泳迁移特性；与碘或 2-巯基乙醇反应后，通过蛋白质印迹转移评估，蛋白质 III 的抗原性未改变。这些数据证明外膜蛋白III是多种淋球菌菌株共有的表面暴露的抗原部分。
Some properties of gonococcal outer membrane III were studied by using a monoclonal antibody (2E6) in Western blot transfer and immunoprecipitation reactions. By Western blot, the 2E6 monoclonal had a single antigenic target: outer membrane protein III. All three gonococcal strains examined exhibited identical reactivities for their protein(s) III with this monoclonal. When 125I-labeled gonococci were incubated with monoclonal antibody 2E6, lysed in Zwittergent, and antibody-antigen complexes were immunoprecipitated with protein A-Sepharose, both outer membrane proteins I and III were present in the immunoprecipitates regardless of the gonococcal strain used. Exposure of gonococci to Iodogen and 2-mercaptoethanol influences the electrophoretic migration characteristics of protein(s) III in polyacrylamide gel electrophoresis with sodium dodecyl sulfate; the antigenicity of protein(s) III was unaltered when assessed by Western blot transfer after reaction with Iodogen or 2-mercaptoethanol. These data demonstrate that outer membrane protein III is a surface-exposed antigenic moiety common to diverse strains of gonococci.