Cholinergic nerve fibers in bone defects of a rat osteoporosis model and their regulation by implantation of bone substitution materials.
Cholinergic nerve fibers in bone defects of a rat osteoporosis model and their regulation by implantation of bone substitution materials.
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发表时间：
2014-06
影响因子：
1.9
通讯作者：
K. Lips;V. Kauschke;S. Hartmann;U. Thormann;S. Ray;M. Schumacher;M. Gelinsky;S. Heinemann;T. Hanke;A. Kautz;M. Schnabelrauch;G. Szalay;C. Heiss;R. Schnettler;V. Alt;O. Kilian
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作者：
K. Lips;V. Kauschke;S. Hartmann;U. Thormann;S. Ray;M. Schumacher;M. Gelinsky;S. Heinemann;T. Hanke;A. Kautz;M. Schnabelrauch;G. Szalay;C. Heiss;R. Schnettler;V. Alt;O. Kilian
自主神经骨由自主神经系统支配，自主神经系统由最近在骨中发现的交感神经和副交感神经组成。因此，我们询问副交感神经是否出现在骨质疏松症动物模型中的骨缺损和植入用于稳定和改善愈合的替代材料的界面处。方法采用去卵巢和缺食的方法建立大鼠骨质疏松模型。在股骨干骺端区域进行楔形截骨术。植入了8种不同的植入物，这些植入物基于磷酸钙骨水泥、铁、二氧化硅矿化胶原和锶改性。以抗囊泡乙酰胆碱转运体（VAChT）、酪氨酸羟化酶（TH）和蛋白基因产物9.5（PGP 9.5）的抗体作为神经元标记物，通过免疫组织化学对神经进行鉴定。结果在肉芽组织填充的缺损区及周围的肥大细胞中，均可见VAChT免疫组化标记的胆碱能神经。植入后未发现胆碱能神经的免疫标记。如PGP 9.5所示，植入后神经的总体存在减少。通过TH免疫标记鉴定的交感神经在锶功能化材料中增加。结论由于植入后胆碱能神经支配减弱，进一步增加替代材料与神经的相容性可以改善缺损愈合，尤其是骨质疏松骨的缺损愈合。
OBJECTIVES Bone is innervated by autonomic nervous system that consists of sympathetic and parasympathetic nerves that were recently identified in bone. Thus we asked whether parasympathetic nerves occur in bone defects and at the interface of substitution materials that were implanted for stabilization and improvement of healing in an osteoporosis animal model. METHODS Osteoporosis was induced in rats by ovariectomy and deficiency diet. A wedge-shaped osteotomy was performed in the metaphyseal area of femur. Eight different implants were inserted that were based on calcium phosphate cement, iron, silica-mineralized collagen, and modifications with strontium. Nerves were identified by immunohistochemistry with antibodies against vesicular acetylcholine transporter (VAChT), tyrosine hydroxylase (TH) and protein gene product 9.5 (PGP 9.5) as neuronal marker. RESULTS Cholinergic nerves identified with VAChT immunostaining were detected in defects filled with granulation tissue and in surrounding mast cells. No immunolabeling of cholinergic nerves was found after implantation. The general presence of nerves was reduced after implantation as shown by PGP 9.5. Sympathetic nerves identified by TH immunolabeling were increased in strontium functionalized materials. CONCLUSION Since cholinergic innervation was diminished after implantation a further increase in the compatibility of substitution materials to nerves could improve defect healing especially in osteoporotic bone.