Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes.
Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes.
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DOI：
10.1186/gb-2006-7-8-r76
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发表时间：
2006
期刊：
影响因子：
12.3
通讯作者：
Stitt M
中科院分区：
文献类型：
--
作者：
Gibon Y;Usadel B;Blaesing OE;Kamlage B;Hoehne M;Trethewey R;Stitt M
对拟南芥叶片昼夜周期中137种代谢物的代谢物和转录水平以及酶活性的时间动态分析全基因组转录谱分析和对中心碳和氮代谢酶活性的分析表明，转录水平在昼夜周期期间和转移到黑暗后发生显着且快速的变化，而活性的变化较小且延迟。在无淀粉的pgm突变体中，每晚糖都被耗尽，转录水平的昼夜变化加剧。这种突变体中的酶活性没有表现出较大的昼夜变化;相反，它们在几天的黑暗后向野生型中发现的水平转移。这表明酶活性变化缓慢，整合了几个昼夜循环中转录水平的变化。为了概括这一结论，使用气相色谱和液相色谱结合质谱分析了137种代谢物。pgm代谢物水平的昼夜变化幅度（糖除外）与野生型相似或小于野生型。平均水平向野生型在黑暗中几天后发现的水平转移。实例包括由于蛋白质降解导致的氨基酸水平增加、脂肪酸水平降低、生育酚增加和肌醇减少。在无淀粉突变体中发现了许多代谢产物与转录本的相关性，并且与糖相关的转录本比例显著增加。转录水平的快速昼夜变化随着时间的推移被整合，以在代谢的大部分中产生准稳定的变化。这意味着代谢物和转录物之间的相关性是由于代谢物对基因表达的调节，而不是由于基因表达的变化而改变代谢物。
An analysis of the temporal dynamics of metabolite and transcript levels, as well as enzyme activity, of 137 metabolites during diurnal cycles in Arabidopsis leaves Genome-wide transcript profiling and analyses of enzyme activities from central carbon and nitrogen metabolism show that transcript levels undergo marked and rapid changes during diurnal cycles and after transfer to darkness, whereas changes in activities are smaller and delayed. In the starchless pgm mutant, where sugars are depleted every night, there are accentuated diurnal changes in transcript levels. Enzyme activities in this mutant do not show larger diurnal changes; instead, they shift towards the levels found in the wild type after several days of darkness. This indicates that enzyme activities change slowly, integrating the changes in transcript levels over several diurnal cycles. To generalize this conclusion, 137 metabolites were profiled using gas and liquid chromatography coupled to mass spectroscopy. The amplitudes of the diurnal changes in metabolite levels in pgm were (with the exception of sugars) similar or smaller than in the wild type. The average levels shifted towards those found after several days of darkness in the wild type. Examples include increased levels of amino acids due to protein degradation, decreased levels of fatty acids, increased tocopherol and decreased myo-inositol. Many metabolite-transcript correlations were found and the proportion of transcripts correlated with sugars increased dramatically in the starchless mutant. Rapid diurnal changes in transcript levels are integrated over time to generate quasi-stable changes across large sectors of metabolism. This implies that correlations between metabolites and transcripts are due to regulation of gene expression by metabolites, rather than metabolites being changed as a consequence of a change in gene expression.