Development and implementation of a three-choice serial reaction time task for zebrafish (Danio rerio)
Development and implementation of a three-choice serial reaction time task for zebrafish (Danio rerio)
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DOI：
10.1016/j.bbr.2011.10.037
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发表时间：
2012-02-01
影响因子：
2.7
通讯作者：
Brennan, Caroline H.
中科院分区：
文献类型：
--
作者：
Parker, Matthew O.;Millington, Mollie E.;Brennan, Caroline H.
斑马鱼是一种成熟的和广泛使用的发育遗传模型系统，但在发达的行为测定的局限性，这意味着他们的潜力作为一个模型在行为神经科学尚未完全实现。在这里，我们描述了一种新的操作性行为测定的发展，研究了斑马鱼的刺激控制的各个方面，使用3选择连续反应时间任务（3 CSRTT）。鱼被短暂地暴露于三个空间上不同的，但感知相同的刺激，在一个固定的时间间隔（ITI）后，以随机顺序呈现。无论是在刺激呈现期间，还是在呈现后的短暂有限保持期内，对正确反应孔径的响应都通过杂志灯的照明和小食物奖励的递送来加强。训练后，过早反应进行了探测与长ITI会议三次;一次在基线，一次在盐水注射后，一次在注射低剂量的安非他明（AMPH; 0.025毫克/公斤）。我们预测，如果过早反应与冲动有关（如啮齿动物），则在注射AMPH后会减少。结果证实，斑马鱼可以学习执行复杂的操作性任务，类似于为啮齿动物开发的用于探测持续注意力和冲动的任务，但AMPH试验的结果并不确定。这项研究为开发和进一步验证该物种作为人类注意力和冲动控制障碍（如药物滥用障碍）某些方面的模型提供了基础。(C)2011 Elsevier B. V.保留所有权利。
Zebrafish are an established and widely utilized developmental genetic model system, but limitations in developed behavioral assays have meant that their potential as a model in behavioral neuroscience has yet to be fully realized. Here, we describe the development of a novel operant behavioral assay to examine a variety of aspects of stimulus control in zebrafish using a 3 choice serial reaction time task (3 CSRTT). Fish were briefly exposed to three spatially distinct, but perceptually identical stimuli, presented in a random order after a fixed-time inter-trial interval (ITI). Entries to the correct response aperture either during the stimulus presentation, or within a brief limited hold period following presentation, were reinforced with illumination of the magazine light and delivery of a small food reward. Following training, premature responding was probed with a long-ITI session three times; once at baseline, once following a saline injection and once following an injection of a low dose of amphetamine (AMPH; 0.025 mg/kg). We predicted that if premature responding was related to impulsivity (as in rodents) it would be reduced following the AMPH injection. Results confirmed that zebrafish could learn to perform a complex operant task similar to tasks developed for rodents which are used to probe sustained attention and impulsivity, but the results from the AMPH trials were inconclusive. This study provides the foundations for development and further validation of this species as a model for some aspects of human attentional and impulse control disorders, such as substance abuse disorder. (C) 2011 Elsevier B.V. All rights reserved.