Soy phytochemicals decrease nonsmall cell lung cancer growth in female athymic mice
Soy phytochemicals decrease nonsmall cell lung cancer growth in female athymic mice
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DOI：
10.1093/jn/138.7.1360
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发表时间：
2008-07-01
影响因子：
4.2
通讯作者：
Scambia, Giovanni
中科院分区：
文献类型：
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作者：
Gallo, Daniela;Zannoni, Gian Franco;Scambia, Giovanni
本研究探讨了含植物雌激素的标准化大豆提取物（SSE）对雌性无胸腺小鼠非小细胞肺癌（NSCLC; A549）异种移植瘤生长的影响。荷瘤小鼠接受无菌水或SSE [50或100 mg/（kg.d），口服]，5 d/周，直至各组的平均肿瘤重量至少为900 mg。与对照组相比，用SSE处理降低了高剂量组的肿瘤生长（P< 0.01）;与对照组相比，两个处理组中的肿瘤具有降低的增殖和更大的凋亡（P < 0.05）。SSE治疗还诱导了弥漫性中央坏死，减少了肿瘤内的活组织块。尽管对照组和治疗组小鼠肿瘤中表皮生长因子受体的水平相当，但100 mg SSE/（kg.d）治疗组小鼠肿瘤中磷酸化蛋白激酶B（p-Akt）的表达低于对照组（P < 0.01）。磷酸化丝裂原活化蛋白激酶的蛋白水平也倾向于在该组中低于对照组（P = 0.07）。雌激素受体（ER）α和ER β存在于肿瘤中，但它们的表达水平在各组之间没有差异。血清胰岛素样生长因子-1浓度也不受治疗的影响。总之，我们发现大豆植物化学物质减缓了NSCLC异种移植物的体内生长;在SSE治疗小鼠肿瘤中观察到的Akt信号通路的调节可能在观察到的活性中起作用。我们的研究进一步支持了植物雌激素的消费可能有效延缓肺癌进展的概念。
This study investigated the effects of a phytoestrogen-containing standardized soy extract (SSE) on the growth of nonsmall cell lung cancer (NSCLC; A549) xenografts in female athymic mice. Tumor-bearing mice received either sterile water or SSE [50 or 100 mg/(kg.d), per os], 5 d/wk, until the mean tumor weight in each group was at least 900 mg. Treatment with SSE reduced tumor growth in the high-dose group compared with control (P< 0.01); tumors in both treated groups had reduced proliferation and greater apoptosis compared with controls (P < 0.05). SSE treatment also induced diffuse central necrosis, reducing the viable tissue mass within the tumor. Whereas tumor levels of epidermal growth factor receptor were comparable in control and treated mice, the expression of phosphorylated protein kinase B (p-Akt) was lower in tumors of mice treated with 100 mg SSE/(kg.d) than in controls (P < 0.01). The protein level of phosphorylated mitogen-activated protein kinase also tended to be lower (P = 0.07) in this group than in controls. Estrogen receptor (ER)alpha and ER beta were present in tumors, but their expression levels did not differ among groups. Serum insulin-like growth factor-1 concentrations also were not affected by the treatments. In conclusion, we found that soy phytochemicals slow the in vivo growth of NSCLC xenografts; the modulation of the Akt-signaling pathway observed in tumors of SSE-treated mice may have a role in the activity observed. Our research provides further support for the concept that consumption of phytoestrogens may be effective in delaying lung cancer progression.