Characterization of a Gene Encoding KK-42-Binding Protein in Antheraea pernyi (Lepidoptera: Saturniidae)
Characterization of a Gene Encoding KK-42-Binding Protein in Antheraea pernyi (Lepidoptera: Saturniidae)
复制标题
柞蚕（鳞翅目：土星科）编码 KK-42 结合蛋白的基因的表征
DOI：
10.1603/an12009
复制
发表时间：
2012-09-01
影响因子：
2.3
通讯作者：
Jiang, De-Fu
中科院分区：
文献类型：
--
作者：
Liu, Yan-Qun;Chen, Miao-Miao;Jiang, De-Fu
KK-42是一种咪唑类昆虫生长调节剂。一种（KK-42）结合蛋白（KK-42 BP）与天蚕（Antheraea yamai）（Guérin-Méneville）（鳞翅目：天蚕科）幼虫的滞育终止有关。在这项研究中，（KK-42）-结合蛋白基因（ApKK-42 BP）的特点是从柞蚕（Guérin-Méneville）（鳞翅目：土星科）经历冬季滞育作为蛹。同源性比较表明，来自pernyi和A. yamai的KK-42 BP与来自鳞翅目昆虫家蚕的已知次要卵黄蛋白密切相关，Plodia interpunctella（Hübner）和Galleria mellonella L.这两个KK-42 BP还含有脂肪酶样区域，如在已知的鳞翅目卵黄蛋白中所观察到的。系统进化分析表明，KK-42 BP是卵黄蛋白中的一个新成员。逆转录-聚合酶链反应分析表明，ApKK-42 BP mRNA的表达在所有的组织测试，在整个四个发展阶段，在女性和男性。在脑和血淋巴中，ApKK-42 BP mRNA的表达水平在非滞育和滞育的幼虫个体之间是相似的。ApKK-42 BPmRNA在滞育蛹的滞育前期表达，滞育前期和滞育期消失，滞育后期又出现。这些表达模式表明，KK-42 BP的缺乏与蛹滞育有关，其表达可能对非滞育个体至关重要。我们的研究结果表明，KK-42 BP基因可能参与的功能以外的蛋黄蛋白。
KK-42 is an imidazole insect growth regulator. A (KK-42)-binding protein (KK-42BP) has been shown to be associated with diapause termination in pharate first instars of Antheraea yamamai (Guérin-Méneville) (Lepidoptera: Saturniidae). In this study, the (KK-42)-binding protein gene (ApKK-42BP) was characterized from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae) undergoing a winter diapause as a pupa. Homologous comparison revealed that KK-42BPs from A.pernyi and A.yamamai were closely related to the known minor yolk proteins from the lepidopterans Bombyx mori L., Plodia interpunctella (Hübner), and Galleria mellonella L. The two KK-42BPs also contained a lipase-like region, as observed in the known lepidopteran yolk proteins. Phylogenetic analysis suggested that KK-42BP is a new member of the minor yolk proteins. Reverse transcription-polymerase chain reaction analyses showed that ApKK-42BP mRNA was expressed in all of the tissues tested, throughout four developmental stages, and in both female and male. Both in the brain and hemolymph, expression of ApKK-42BP mRNA level was similar between nondiapause-destined and diapause-destined larval individuals. ApKK-42BP mRNA was expressed in the predia-pause period of diapause-destined pupae, disappeared in early diapause and diapause stage, and reappeared in the postdiapause stage. These expression patterns stated that a lack of KK-42BP is associated with pupal diapause and its expression may be critical to nondiapausing individuals. Our results suggested that the KK-42BP gene is likely involved in a function other than as a yolk protein.